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Purified anti-β-Amyloid, 1-42 Antibody (Prev
产品名称：
Purified anti-β-Amyloid, 1-42 Antibody (Prev
产品类别：
抗体
产品编号：
805509
产品应用：
805509
[价格]
规格 价格 库存
50µL ￥ 1764 1

产品详情

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P -?Qualty tested
Direct ELISA, WB -?Verified
IHC-F, ELISA -?Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.2 - 5.0 ?g/ml is suggested. For Direct ELISA,?a concentration range of 0.015 - 1.5 ?g/ml is suggested.?For Western blotting, the suggested use of this reagent is 1.0 - 5.0 ?g/ml.?It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), and ELISA.

This antibody is reactive to the C-terminus of beta amyloid and is specific for the isoform ending at the 42nd amino acid.

Additional reported applications (for the relevant formats) includes: immunohistochemical staining of paraformaldehyde-fixed frozen sections6 and ELISA3,4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Helwig M,?et al. 2013.?J. Biol. Chem.?288:1114.?(IHC-P)
  2. Takami M,?et al.2009.?J. Neurosci.; 29: 13042.?(IHC-P)
  3. Cenini G,?et al.?2012.?Biochim Biophys Acta.?1822(2):130.?(ELISA)?PubMed
  4. Sundaram RK,?et al.?2012.?Int. J. Pept. Res. Ther.?18(2):99.?(ELISA)?PubMed
  5. Savage MJ,?et al.?2014.?J. Neurosci.?8:2884.?(ELISA)?PubMed
  6. Mastrangelo MA, Bowers WJ. 2008.?BMC Neurosci.?9:81.?(IHC-F)?PubMed
Product Citations
  1. Barbato C, et al. 2020. Molecular Neurobiology. 57(7):3183-3194. PubMed
  2. Nick H, et al. 2022. Front Aging Neurosci. 14:1025402. PubMed
  3. Vingtdeux V, et al. 2011. FASEB J. 25:219-231. PubMed
  4. Kim C, et al. 2017. Mol Neurobiol. 10.1007/s12035-017-0683-3. PubMed
  5. Townsend M, et al. 2010. J Pharmacol Exp Ther. 333:110:119. PubMed
  6. Ruiz-Riquelme A, et al. 2021. Acta Neuropathol Commun. 9:83. PubMed
  7. Helwig M, et al. 2013. J Biol Chem. 288:1114-1124. PubMed
  8. Moreno F, et al. 2017. PLoS One. 12(6):e0178093. PubMed
  9. Koh HS, et al. 2021. Int J Mol Sci. 22:. PubMed
  10. Gutknecht MF, et al. 2022. Cell Rep Methods. 2:100214. PubMed
  11. Owen JE, et al. 2021. Sleep. 44:zsab057. PubMed
  12. Kim H, et al. 2021. Front Aging Neurosci. 13:709091. PubMed
  13. Olah M, et al. 2020. Nat Commun. 4.714583333. PubMed
  14. Augé E, et al. 2017. Sci Rep. 7:41807. PubMed
  15. Shi H, et al. 2020. Acta Neuropathol. 139:813. PubMed
  16. Takami M, et al. 2009. J Neurosci. 29:13042-13052. PubMed
  17. Maheshwari M, et al. 2010. Biochemistry. 49:10371-10380. PubMed
  18. Sudol K, et al. 2009. Mol Ther. 17:2031-2040. PubMed
  19. Lee CYD et al. 2018. Neuron. 97(5):1032-1048 . PubMed
  20. Plowey ED, et al. 2022. Acta Neuropathol. 144:143. PubMed
  21. Kim K, et al. 2020. Nat Commun. 0.540972222. PubMed
  22. Chan HN, et al. 2019. Theranostics. 9:2939. PubMed
  23. Kommaddi RP et al. 2018. The Journal of Neuroscience. 38(5):1085-1099 . PubMed
  24. Piccirillo S, et al. 2022. Cell Death Discov. 8:391. PubMed
  25. Li K, et al. 2020. ACS Chemical Neuroscience. 11(24):4316-4328. PubMed
  26. Ohmi K, et al. 2011. PLoS One. 6:e27461. PubMed
  27. Vassar W 2013. Mol Neurodegener. 8:2. PubMed
  28. Faraz A, et al. 2017. Antioxid Redox Signal. . 10.1089/ars.2016.6860. PubMed
  29. Maxwell AM, et al. 2021. Acta Neuropathol Commun. 9:201. PubMed
  30. Parker H, et al. 2020. EMBO Mol Med. 12:e11185. PubMed
  31. Ruiz–Riquelme A, et al. 2018. Acta Neuropathol Commun. 6:26. PubMed
RRID
AB_2783381 (BioLegend Cat. No. 805509) AB_2564683 (BioLegend Cat. No. 805501) AB_2564684 (BioLegend Cat. No. 805502) AB_2564682 (BioLegend Cat. No. 805503)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue sources: Primarily nervous system, but also adipose tissue, intestine, muscle.?
Distribution: cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A,?et al.?2015. Pharmacol. Rep. 67(2):195.
  2. Sadigh-Eteghad S,?et al. 2015. Med. Princ. Pract. 24(1):1
  3. Hampel H,?et al.?2015.?Expert Rev. Neurother.?15(1):83.
  4. Puig KL,?et al.?2012. ?Exp. Gerontol. 48(7): 608.
  5. Selkoe DJ,?et al.?2016.?EMBO Mol. Med.?8(6):595.
  6. Walsh DM,?et al.??2007.?J. Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid 1-42 on UniProt.org

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