HRP anti-β-Amyloid, 1-42 Antibody (Previously-上海雅吉生物科技有限公司

规格	价格	库存
200µL	￥ 7596	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Aqueous buffered solution containing a carrier protein and preservatives.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

Store between 2°C and 8°C.

Application

IHC-P - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 ?g/ml is suggested.?For Western blotting, the suggested use of this reagent is 1.0 - 10 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in ELISA, Immunoblotting (WB) and Immunohistochemistry on?frozen and?formalin-fixed paraffin-embedded tissues (IHC-P, IHC-F).

Reactive to the c-terminus of beta amyloid and specific for the isoform ending at the 42nd amino acid.

Additional reported applications (for the relevant formats) includes: immunohistochemical staining of paraformaldehyde-fixed frozen sections⁶ and ELISA^3,4,5.

Application References (PubMed link indicates BioLegend citation)

Helwig M,?et al. 2013.?J. Biol. Chem.?288:1114.?(IHC-P)
Takami M,?et al.2009.?J. Neurosci.; 29: 13042.?(IHC-P)
Cenini G,?et al.?2012.?Biochim Biophys Acta.?1822(2):130.?(ELISA)?PubMed
Sundaram RK,?et al.?2012.?Int. J. Pept. Res. Ther.?18(2):99.?(ELISA)?PubMed
Savage MJ,?et al.?2014.?J. Neurosci.?8:2884.?(ELISA)?PubMed
Mastrangelo MA, Bowers WJ. 2008.?BMC Neurosci.?9:81.?(IHC-F)?PubMed

Product Citations

Casali BT, et al. 2020. Neurobiol Dis. 142:104956. PubMed
Cheng-Hathaway PJ, et al. 2018. Mol Neurodegener. 13:e0200060. PubMed

RRID

AB_2564980 (BioLegend Cat. No. 805507)

Antigen Details

Structure

Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.

Distribution

Tissue sources: Primarily nervous system, but also adipose tissue, intestine, muscle.?
Distribution: cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function

The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.

Biology Area

Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation

Molecular Family

APP/β-Amyloid

Antigen References