Ultra-LEAF™ Purified anti-mouse IL-4 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1416	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Partially purified native mouse IL-4

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
CyTOF? - Verified
ELISPOT Capture, Neut, ICC, IP, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISPOT applications, a concentration range of 2-6 μg/ml is recommended. For ELISA capture applications, a concentration range of 0.5-2.0 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-4 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA^1,2,10,13 or ELISPOT⁵ Capture: The purified 11B11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD6-24G2 antibody (Cat. No. 504202) as the detecting antibody and recombinant mouse IL-4 (Cat. No. 575609) as the standard. The LEAF? purified antibody is suggested for ELISPOT capture.
Neutralization^1-2,9,12: The 11B11 antibody can neutralize the bioactivity of natural or recombinant IL-4. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo and in vitro (Cat. No. 504108).
Additional reported applications (for the relevant formats) include: immunoprecipitation¹⁶, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections⁸ and paraformaldehyde-fixed, saponin-treated frozen tissue sections^6,7, and immunocytochemistry⁴.
Note: For testing mouse IL-4 in serum, plasma or supernatant, BioLegend's ELISA Max? Sets (Cat. No. 431101 to 431106) are specially developed and recommended.

Additional Product Notes

Get a 50% discount on this product when purchased in our Activation Bundles. Restrictions apply. Learn more…

Application References (PubMed link indicates BioLegend citation)

Shirai A, et al. 1994. Cytokine 6:329. (ELISA, Neut)
Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA, Neut)
Assenmacher M, et al. 1994. Eur. J. Immunol. 24:1097.
Openshaw P, et al. 1995. J. Exp. Med. 182:1357. (ICC)
Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISA Capture)
Litton M, et al. 1994. J. Immunol. Methods 175:47. (IHC)
Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
Hara M, et al. 2001. J. Immunol. 166:3789. (Neut)
Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
Lawson BR, et al. 2007. J. Immunol. 178:5366.
Wang W, et al. 2007. J. Immunol. 178:4885. (Neut)
Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
Ohnmacht C, et al. 2008. Blood 113:2816. PubMed
Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
Zavorotinskaya T, et al. 2003. Mol. Ther. 7:155. (IP)

Product Citations

Kenichi Shimada et al. 2018. Immunity. 49(5):873-885 . PubMed
Ogbechi J, et al. 2022. Front Immunol. 13:1001956. PubMed
Imanishi T, et al. 2020. Cell Reports. 32(3):107911. PubMed
Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
Jia X, et al. 2021. Front Cell Dev Biol. 9:685522. PubMed
Montes de Oca M, et al. 2020. PLoS Pathog. 16:e1008994. PubMed
Guo P, et al. 2021. J Immunol. 207:408. PubMed
Kanno T, et al. 2022. Front Immunol. 13:904875. PubMed
Xia X, et al. 2020. J Clin Invest. 130:5180. PubMed
Price C, et al. 2020. Cell Host & Microbe. 27(4):571-584.e7.. PubMed
Hao L, et al. 2021. Br J Pharmacol. 178:4726. PubMed
Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
Ding X, et al. 2021. Front Immunol. 12:667136. PubMed

RRID

AB_11149679 (BioLegend Cat. No. 504121) AB_11150601 (BioLegend Cat. No. 504122) AB_2750404 (BioLegend Cat. No. 504135) AB_2750405 (BioLegend Cat. No. 504136) AB_2750406 (BioLegend Cat. No. 504137) AB_2750407 (BioLegend Cat. No. 504138)

Antigen Details

Structure: Cytokine; 15-19 kD (Mammalian)
Bioactivity: Differentiation of na?ve CD4⁺ T cells to the T_H2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources: Mast cells, T cells, bone marrow stromal cells
Cell Targets: B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors: Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type: Tregs
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.
Regulation: Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID: 16189 View all products for this Gene ID
UniProt: View information about IL-4 on UniProt.org

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