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PE/Cyanine7 anti-mouse IL-4 Antibody
产品名称：
PE/Cyanine7 anti-mouse IL-4 Antibody
产品类别：
抗体
产品编号：
504117
产品应用：
504117
[价格]
规格 价格 库存
25µg ￥ 1548 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partially purified native mouse IL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 ?g per million cells in 100 ?l volume.? It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA1,2,10,13 or ELISPOT5 Capture: The purified 11B11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD6-24G2 antibody (Cat. No. 504202) as the detecting antibody and recombinant mouse IL-4 (Cat. No. 575609) as the standard. The LEAF? purified antibody is suggested for ELISPOT capture.
Neutralization1-2,9,12: The 11B11 antibody can neutralize the bioactivity of natural or recombinant IL-4. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo and in vitro (Cat. No. 504108).
Additional reported applications (for the relevant formats) include: immunoprecipitation16, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections6,7, and immunocytochemistry4.
Note: For testing mouse IL-4 in serum, plasma or supernatant, BioLegend's ELISA Max? Sets (Cat. No. 431101 to 431106) are specially developed and recommended.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Shirai A, et al. 1994. Cytokine 6:329. (ELISA, Neut)
  2. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA, Neut)
  3. Assenmacher M, et al. 1994. Eur. J. Immunol. 24:1097.
  4. Openshaw P, et al. 1995. J. Exp. Med. 182:1357. (ICC)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISA Capture)
  6. Litton M, et al. 1994. J. Immunol. Methods 175:47. (IHC)
  7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
  8. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  9. Hara M, et al. 2001. J. Immunol. 166:3789. (Neut)
  10. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
  11. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  12. Wang W, et al. 2007. J. Immunol. 178:4885. (Neut)
  13. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
  14. Ohnmacht C, et al. 2008. Blood 113:2816. PubMed
  15. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  16. Zavorotinskaya T, et al. 2003. Mol. Ther. 7:155. (IP)
Product Citations
  1. Kisielow J, et al. 2019. Nat Immunol. 1.286111111. PubMed
  2. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  3. Shibata K, et al. 2014. J Immunol. 192:2210. PubMed
  4. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  5. Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed
  6. Wang W, et al. 2020. Br J Pharmacol. 177:5569. PubMed
  7. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  8. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  9. Alves C, et al. 2015. PLoS One. 10: 0142972. PubMed
  10. Cho S, et al. 2017. Sci Rep. 7:35. PubMed
  11. Dai G, et al. 2021. mSphere. 6: . PubMed
  12. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  13. Jiang J, et al. 2017. Arthritis Res Ther. 10.1186/s13075-017-1261-9. PubMed
  14. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  15. Morimoto Y, et al. 2018. Immunity. 49:134. PubMed
  16. Ryan NM, et al. 2022. Front Immunol. 13:932742. PubMed
  17. Kim DK, et al. 2022. Nat Commun. 13:6292. PubMed
  18. Garcia-Dominguez D, et al. 2022. Front Immunol. 13:948335. PubMed
  19. Voigt A, et al. 2015. Biol Open. 10.1242/bio.013771. PubMed
  20. Wang Y, et al. 2021. Front Immunol. 11:601601. PubMed
  21. Smith R, et al. 2016. J Leukoc Biol. 100:371-80. PubMed
  22. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  23. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
RRID
AB_10895747 (BioLegend Cat. No. 504117) AB_10898116 (BioLegend Cat. No. 504118)

Antigen Details

Structure
Cytokine; 15-19 kD (Mammalian)
Bioactivity
Differentiation of na?ve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources
Mast cells, T cells, bone marrow stromal cells
Cell Targets
B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors
Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.

Regulation
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID
16189 View all products for this Gene ID
UniProt
View information about IL-4 on UniProt.org

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