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首页 > 抗体蛋白 > 抗体
PE anti-mouse IL-4 Antibody
产品名称：
PE anti-mouse IL-4 Antibody
产品类别：
抗体
产品编号：
504103
产品应用：
504103
[价格]
规格 价格 库存
25µg ￥ 1056 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partially purified native mouse IL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA1,2,10,13 or ELISPOT5 Capture: The purified 11B11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD6-24G2 antibody (Cat. No. 504202) as the detecting antibody and recombinant mouse IL-4 (Cat. No. 575609) as the standard. The LEAF? purified antibody is suggested for ELISPOT capture.
Neutralization1-2,9,12: The 11B11 antibody can neutralize the bioactivity of natural or recombinant IL-4. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo and in vitro (Cat. No. 504108).
Additional reported applications (for the relevant formats) include: immunoprecipitation16, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections6,7, and immunocytochemistry4.
Note: For testing mouse IL-4 in serum, plasma or supernatant, BioLegend's ELISA Max? Sets (Cat. No. 431101 to 431106) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Shirai A, et al. 1994. Cytokine 6:329. (ELISA, Neut)
  2. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA, Neut)
  3. Assenmacher M, et al. 1994. Eur. J. Immunol. 24:1097.
  4. Openshaw P, et al. 1995. J. Exp. Med. 182:1357. (ICC)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISA Capture)
  6. Litton M, et al. 1994. J. Immunol. Methods 175:47. (IHC)
  7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
  8. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  9. Hara M, et al. 2001. J. Immunol. 166:3789. (Neut)
  10. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
  11. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  12. Wang W, et al. 2007. J. Immunol. 178:4885. (Neut)
  13. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
  14. Ohnmacht C, et al. 2008. Blood 113:2816. PubMed
  15. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  16. Zavorotinskaya T, et al. 2003. Mol. Ther. 7:155. (IP)
Product Citations
  1. Kim K, et al. 2016. PLoS One. 11: 0148576. PubMed
  2. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  3. Dai L, et al. 2020. Cell. 182(3):722-733.e11. PubMed
  4. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  5. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  6. Gerber AN, et al. 2021. Cell Rep. 37:109816. PubMed
  7. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
  8. Lua Y, et al. 2012. Mol Immunol. 52:229. PubMed
  9. Su N, Xiong Y 2016. Sci Rep. 6:22658. PubMed
  10. Sanchez-Felipe L, et al. 2021. Nature. 590:320. PubMed
  11. Wei H, et al. 2021. Malar J. 20:89. PubMed
  12. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  13. Amir M, et al. 2018. Cell Rep. 25:3733. PubMed
  14. Lu Y, et al. 2019. Int J Mol Med. 43:2398. PubMed
  15. Zhang C, et al. 2022. J Extracell Vesicles. 11:e12209. PubMed
  16. Volpedo G, et al. 2022. NPJ Vaccines. 7:32. PubMed
  17. Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed
  18. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  19. Kohu K, et al. 2009. J Immunol. 183:7817. PubMed
  20. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  21. Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed
  22. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  23. Green CD et al. 2018. Developmental cell. 46(5):651-667 . PubMed
  24. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  25. Wu G, et al. 2020. Br J Pharmacol. 177:5063. PubMed
  26. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  27. Shiomi A, et al. 2014. J Immunol. 193:849. PubMed
  28. Sawant D, et al. 2014. J Immunol. 192:2904. PubMed
  29. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  30. Ikeda U, et al. 2010. Immunol Lett. 134:7. PubMed
  31. Bhattacharya D, et al. 2014. J Biol Chem. 289:16508. PubMed
  32. Bogie JF, et al. 2020. Ther Adv Chronic Dis. 11:2040622320947378. PubMed
  33. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  34. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  35. Coe D, et al. 2022. JCI Insight. 7:. PubMed
  36. Wang Z, et al. 2022. Nat Biomed Eng. 6:791. PubMed
  37. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  38. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  39. Chung NH, et al. 2022. Vaccine. 40:574. PubMed
  40. Yuan Y, et al. 2022. Cell Rep. 38:110256. PubMed
  41. Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed
  42. Kim DH, et al. 2018. Nat Commun. 9:503. PubMed
  43. Yang B, et al. 2022. Bioengineered. 13:2685. PubMed
  44. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  45. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  46. Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
  47. Dudeck J, et al. 2019. J Allergy Clin Immunol. 143:1849. PubMed
  48. Tajima M, et al. 2022. Curr Protoc. 2:e540. PubMed
  49. Choi J, et al. 2015. PLoS One. 10: e0138592. PubMed
  50. Xiao u, et al. 2015. J Immunol. 195: 4218 - 4227. PubMed
  51. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  52. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  53. Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
  54. Chan Y, et al. 2009. J Immunol. 183:7039. PubMed
  55. Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
  56. Tian D, et al. 2019. Nat Commun. 10:4246. PubMed
  57. Ma Q, et al. 2022. Vaccines (Basel). 10:. PubMed
  58. Xu Y, et al. 2021. iScience. 24:103445. PubMed
  59. Huang LJ, et al. 2021. Immunity. 54(8):1728-1744.e7. PubMed
  60. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  61. Mykk?nen O, et al. 2014. PLoS One. 9:114790. PubMed
  62. Zeng J, et al. 2020. Int J Biol Sci. 1.939583333. PubMed
  63. Nomura S, et al. 2019. Sci Rep. 9:17335. PubMed
  64. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  65. Muri J, et al. 2020. Cell Reports. 30(13):4399-4417. PubMed
  66. Brenner E, et al. 2020. Nat Commun. 11:1335. PubMed
  67. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
RRID
AB_315317 (BioLegend Cat. No. 504103) AB_315318 (BioLegend Cat. No. 504104)

Antigen Details

Structure
Cytokine; 15-19 kD (Mammalian)
Bioactivity
Differentiation of na?ve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources
Mast cells, T cells, bone marrow stromal cells
Cell Targets
B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors
Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.

Regulation
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID
16189 View all products for this Gene ID
UniProt
View information about IL-4 on UniProt.org

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