Purified anti-LAT Antibody
- 产品名称：
- Purified anti-LAT Antibody
- 产品类别：
- 抗体
- 产品编号：
- 641102
- 产品应用：
- 641102
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- GST fusion protein corresponding to residues 31-233 of human LAT, cytoplasmic domain.
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store between 2°C and 8°C
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone may recognize a ~80 kD protein of unknown origin in some cell lines and tissue homogenates. It is also predicted to recognize rat LAT due to complete sequence homology between human, mouse, and rat LAT.
- Application References
(PubMed link indicates BioLegend citation) -
- Dorn T, et al. 2007. Blood 109:2346.
- Sloan D, et al. 2006. J. Immunol. 176:1825
- Product Citations
-
- Deswal S, et al. 2011. PLoS One. 6:e22928. PubMed
- Chen EW, et al. 2019. Front Immunol. 10:1718. PubMed
- RRID
- AB_2157732 (BioLegend Cat. No. 641102)
- Structure
- Type III transmembrane protein, MW of 28 kD but expected at 36-38 kD due to post-translational modifications including palmitoylation; multiple isoforms with a predicted MW of 25-28 kD reported.
- Distribution
-
Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
- Function
- LAT is required for signaling through a number of cell surface receptors including TCR, FcγRIII, and FcεRI.
- Interaction
- Interacts directly with GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, LCP2, SHB, SKAP2, MIST,and FcγR1A
- Cell Type
- Mast cells, NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Phospho-Proteins
- Antigen References
-
1. Zhang W et al. 1998. Cell 92:83.
2. Weber JR. et al. 1998. J. Exp. Med. 187:1157
- Regulation
- Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation
- Gene ID
- 27040 View all products for this Gene ID
- UniProt
- View information about LAT on UniProt.org
