Purified anti-mouse CD45.2 Antibody
- 产品名称：
- Purified anti-mouse CD45.2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 109802
- 产品应用：
- 109802
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- B10.S mouse thymocytes and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
Block, IHC-F, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.?
- Application References
(PubMed link indicates BioLegend citation) -
- Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
- Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
- Suzuki K, et al. 2000. Immunity 13:691. (IHC)
- Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
- Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
- Pascal V, et al. 2007. J. Immunol. 179:1751.
- Burman AC, et al. 2007. Blood 110:1064.
- Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
- Phan TG, et al. 2007. Nature Immunol. 8:992.
- Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
- Wen T, et al. 2013. PNAS. 110:6067. PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- Product Citations
-
- Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed
- Ma J, et al. 2012. J Immunol. 188:5337. PubMed
- Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
- Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
- Puiffe ML, et al. 2020. Front Immunol. 11:600012. PubMed
- Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
- Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
- Meers GK, et al. 2018. PLoS One. 13:e0190846. PubMed
- Nakano-Yokomizo T, et al. 2011. J Exp Med. 208:1661. PubMed
- Matsuba S, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01538. PubMed
- Lin WC, et al. 2020. J Clin Invest. 130:157. PubMed
- You X, et al. 2022. Front Genet. 12:790990. PubMed
- Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed
- Kolodkin-Gal D, et al. 2021. Gut. Online ahead of print. PubMed
- Speir M, et al. 2020. Nat Immunol. 21:54. PubMed
- Galbas T, et al. 2017. J Immunol. 198(2):852-861. PubMed
- Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
- Basilico S, et al. 2020. Nat Commun. 11:1407. PubMed
- Lin Z, et al. 2021. Stem Cells. 39:240. PubMed
- Takeuchi Y, et al. 2021. Sci Rep. 11:18679. PubMed
- Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
- Fitzpatrick Z, et al. 2020. Nature. 587:472. PubMed
- Udumula MP, et al. 2021. Mol Metab. 53:101272. PubMed
- Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
- Alam IS, et al. 2020. Cancer Res. 80:4780. PubMed
- RRID
- AB_313439 (BioLegend Cat. No. 109802)
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Suzuki K, et al. 2000. Immunity 13:691.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45.2 on UniProt.org
