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首页 > 抗体蛋白 > 抗体
PerCP/Cyanine5.5 anti-mouse CD45.2 Antibody
产品名称：
PerCP/Cyanine5.5 anti-mouse CD45.2 Antibody
产品类别：
抗体
产品编号：
109827
产品应用：
109827
[价格]
规格 价格 库存
25µg ￥ 1008 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.?

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Wang Y, et al. 2021. Cancer Res. 81:174. PubMed
  2. Charpentier JC, et al. 2020. Nat Commun. 11:180. PubMed
  3. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  4. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  5. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  6. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  7. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
  8. Emal D, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2016030255. PubMed
  9. Scalzo S, et al. 2022. Int J Nanomedicine. 17:2865. PubMed
  10. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  11. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  12. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  13. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  14. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  15. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  16. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  17. Low MSY, et al. 2019. Cell Reports. 29(9):2634-2645.e5. PubMed
  18. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  19. Rothchild AC, et al. 2020. Bio Protoc. 10:00. PubMed
  20. Kleinholz CL, et al. 2021. Sci Rep. 11:15071. PubMed
  21. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  22. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  23. Rodriguez-Ruiz M, et al. 2016. Cancer Res . 76: 5994 - 6005. PubMed
  24. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  25. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  26. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  27. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  28. Yap JY, et al. 2018. Immunology. 154:522. PubMed
  29. Tian Y, et al. 2016. J Immunol. 196: 2153 - 2166. PubMed
  30. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  31. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  32. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  33. Zhang K, et al. 2014. PLoS Pathog. 10:1004385. PubMed
  34. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  35. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  36. Xu S, et al. 2021. Immunity. . PubMed
  37. Kan X, et al. 2015. Cardiovasc Res. 10.1093/cvr/cvv255. PubMed
  38. Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
  39. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  40. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  41. Olson GS, et al. 2021. Cell Reports. 35(9):109195. PubMed
  42. Tian Y, et al. 2016. J Immunol. 197: 1308 - 1321. PubMed
  43. Evgin L, et al. 2022. Sci Transl Med. 14:eabn2231. PubMed
  44. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  45. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  46. Giampazolias E, et al. 2021. Cell. . PubMed
  47. MacKinnon A, et al. 2011. Am J Respir Crit Care Med. 185:537. PubMed
  48. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  49. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  50. Wang X, et al. 2016. J Immunol. 197(12):4750-4761. PubMed
  51. Forero A, et al. 2020. Immunity. 51(3):451-464. PubMed
  52. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  53. Tang W, et al. 2021. Front Cell Dev Biol. 9:728057. PubMed
  54. Xu Y, et al. 2021. iScience. 24:103445. PubMed
  55. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  56. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  57. Cao H, et al. 2020. Front Pharmacol. 11:578943. PubMed
  58. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  59. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  60. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  61. Cabal-Hierro L, et al. 2020. Nat Commun. 11:1406. PubMed
RRID
AB_893352 (BioLegend Cat. No. 109827) AB_893350 (BioLegend Cat. No. 109828)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

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