PE/Dazzle™ 594 anti-mouse CD45.2 Antibody
- 产品名称：
- PE/Dazzle™ 594 anti-mouse CD45.2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 109845
- 产品应用：
- 109845
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- B10.S mouse thymocytes and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.?
- Application References
(PubMed link indicates BioLegend citation) -
- Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
- Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
- Suzuki K, et al. 2000. Immunity 13:691. (IHC)
- Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
- Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
- Pascal V, et al. 2007. J. Immunol. 179:1751.
- Burman AC, et al. 2007. Blood 110:1064.
- Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
- Phan TG, et al. 2007. Nature Immunol. 8:992.
- Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
- Wen T, et al. 2013. PNAS. 110:6067. PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- Product Citations
-
- Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
- Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
- Trott DW, et al. 2021. Geroscience. 43:1331. PubMed
- Bowers E, et al. 2018. Nat Med. 24:95. PubMed
- Moreno-Fernandez ME, et al. 2018. JCI Insight. 3. PubMed
- Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
- Morris AB, et al. 2020. Immunity. 52(1):136-150.e6.. PubMed
- Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
- Trott DW, et al. 2021. J Physiol. 599:3973. PubMed
- Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed
- Baja?a S, et al. 2022. iScience. 25:103732. PubMed
- Chew HY, et al. 2020. Cell. 180(5):895-914. PubMed
- S?wen P et al. 2018. eLife. 7 pii: e41258. PubMed
- RRID
- AB_2564176 (BioLegend Cat. No. 109845) AB_2564177 (BioLegend Cat. No. 109846)
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Suzuki K, et al. 2000. Immunity 13:691.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45.2 on UniProt.org
