FITC anti-mouse CD45.2 Antibody
- 产品名称:
- FITC anti-mouse CD45.2 Antibody
- 产品类别:
- 抗体
- 产品编号:
- 109805
- 产品应用:
- 109805
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- B10.S mouse thymocytes and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.?
- Application References
(PubMed link indicates BioLegend citation) -
- Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
- Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
- Suzuki K, et al. 2000. Immunity 13:691. (IHC)
- Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
- Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
- Pascal V, et al. 2007. J. Immunol. 179:1751.
- Burman AC, et al. 2007. Blood 110:1064.
- Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
- Phan TG, et al. 2007. Nature Immunol. 8:992.
- Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
- Wen T, et al. 2013. PNAS. 110:6067. PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- Product Citations
-
- Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
- Miranda K, et al. 2022. iScience. 25:104994. PubMed
- Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
- Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
- Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
- Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
- Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
- Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
- MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
- Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
- Yang X, et al. 2020. Blood Sci. 2:89. PubMed
- Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
- Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
- Burman A, et al. 2007. Blood. 110:1064. PubMed
- Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
- Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
- Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
- Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
- Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
- Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
- Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
- Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
- Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
- Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
- Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
- Zhang J, et al. 2022. iScience. 25:104963. PubMed
- Emg?rd J, et al. 2018. Immunity. 143:419. PubMed
- Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
- Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
- Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
- Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
- Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
- Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
- Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
- Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
- Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
- Glaros V, et al. 2021. Immunity. 54:2005. PubMed
- Li J, et al. 2020. Elife. 9:00. PubMed
- Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
- Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
- Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
- Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
- Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
- Wan X, et al. 2018. Nature. 560:107. PubMed
- Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
- Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
- Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
- Abbott RK, et al. 2018. Immunity. 48:133. PubMed
- Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
- Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
- Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
- Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
- Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
- Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
- Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
- Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
- Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
- Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
- Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
- Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
- Burgess M, et al. 2014. Blood. 124:3947. PubMed
- Kesavardhana S, et al. 2020. J Biol Chem. 295:8325. PubMed
- Zeis P, et al. 2020. Immunity. 53:775. PubMed
- de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
- Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
- Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
- Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
- Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
- Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
- Li E, et al. 2021. Front Immunol. 12:667177. PubMed
- Kissiov DU, et al. 2022. Elife. 11:. PubMed
- Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
- Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
- Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
- Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
- Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
- Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
- Koide S, et al. 2022. iScience. 25:103603. PubMed
- Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
- Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
- Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
- Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
- Field CS, et al. 2020. Cell Metab. 31:422. PubMed
- Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
- Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
- Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
- Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
- Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
- Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
- Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
- Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
- Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
- Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
- Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
- Hung LY, et al. 2019. J Immunol. 203:511. PubMed
- Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
- Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
- Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
- Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
- Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
- Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
- Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
- RRID
- AB_313442 (BioLegend Cat. No. 109805) AB_313443 (BioLegend Cat. No. 109806)
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Suzuki K, et al. 2000. Immunity 13:691.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45.2 on UniProt.org