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Brilliant Violet 421™ anti-mouse CD45.2 Anti
产品名称：
Brilliant Violet 421™ anti-mouse CD45.2 Anti
产品类别：
抗体
产品编号：
109831
产品应用：
109831
[价格]
规格 价格 库存
125µL ￥ 2184 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. For flow cytometric staining using the ?l sizes , the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.?

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Nazet U, et al. 2021. Cells. 10:. PubMed
  2. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  3. Kinder JM, et al. 2020. Cell Reports. 31(12):107784.. PubMed
  4. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  5. Christopher S Garris et al. 2018. Immunity. 49(6):1148-1161 . PubMed
  6. Juttukonda LJ, et al. 2017. Cell Host Microbe. 22:531. PubMed
  7. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  8. Wilkinson AC, et al. 2020. Nature Protocols. 15(2):628-648.. PubMed
  9. Garg G et al. 2019. Cell reports. 26(7):1854-1868 . PubMed
  10. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  11. Wiesner D, et al. 2016. J Immunol. 196: 365 - 374. PubMed
  12. Zebley CC, et al. 2021. Cell Rep. 37:109796. PubMed
  13. Chopin M et al. 2018. Immunity. 50(1):77-90 . PubMed
  14. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  15. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  16. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  17. Crooks SD, et al. 2022. Immunohorizons. 6:705. PubMed
  18. Nazet U, et al. 2021. J Orofac Orthop. Online ahead of print. PubMed
  19. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  20. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  21. Van Braeckel-Budimir N, et al. 2018. Cell Rep. 24:3374. PubMed
  22. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  23. Yang H, et al. 2019. J Neuroinflammation. 16:169. PubMed
  24. Lema?tre F, et al. 2013. J Immunol. 191:1578. PubMed
  25. Zhang X, et al. 2022. JCI Insight. 7:. PubMed
  26. Severance AL, et al. 2022. iScience. 25:104400. PubMed
  27. Fukushima K, et al. 2020. Immunity. 52(3):542-556. PubMed
  28. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  29. Engblom C, et al. 2017. Science. 358:6367. PubMed
  30. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  31. Koide S, et al. 2022. iScience. 25:103603. PubMed
  32. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  33. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  34. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  35. Senatus L, et al. 2020. JCI Insight. 5:00. PubMed
  36. Wilkinson AC, et al. 2019. Nature. 571:117. PubMed
  37. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  38. Song W, et al. 2022. Immunity. 55:290. PubMed
  39. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  40. Tribble JR, et al. 2020. Mol Brain. 13:81. PubMed
  41. Allman W, et al. 2015. Proc Natl Acad Sci U S A. 112: 4094 - 4103. PubMed
RRID
AB_10900256 (BioLegend Cat. No. 109831) AB_2565511 (BioLegend Cat. No. 109832)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

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