Biotin anti-mouse CD45.2 Antibody
- 产品名称：
- Biotin anti-mouse CD45.2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 109803
- 产品应用：
- 109803
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- B10.S mouse thymocytes and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.?
- Application References
(PubMed link indicates BioLegend citation) -
- Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
- Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
- Suzuki K, et al. 2000. Immunity 13:691. (IHC)
- Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
- Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
- Pascal V, et al. 2007. J. Immunol. 179:1751.
- Burman AC, et al. 2007. Blood 110:1064.
- Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
- Phan TG, et al. 2007. Nature Immunol. 8:992.
- Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
- Wen T, et al. 2013. PNAS. 110:6067. PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- Product Citations
-
- McLelland B, et al. 2011. J Immunol Methods. 367:85. PubMed
- Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed
- Yang K, et al. 2018. J Virol. 92:e00428. PubMed
- Fidler TP, et al. 2021. Nature. 592:296. PubMed
- Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
- Zhao L, et al. 2020. EBioMedicine. 60:103020. PubMed
- Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
- Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
- Wolock SL, et al. 2019. Cell Rep. 28:302. PubMed
- Mesa-Nu?ez C, et al. 2022. Mol Ther Methods Clin Dev. 26:459. PubMed
- Kopinke D et al. 2017. Cell. 170(2):340-351 . PubMed
- S?wen P et al. 2018. eLife. 7 pii: e41258. PubMed
- RRID
- AB_313440 (BioLegend Cat. No. 109803) AB_313441 (BioLegend Cat. No. 109804)
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Suzuki K, et al. 2000. Immunity 13:691.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45.2 on UniProt.org
