Alexa Fluor® 488 anti-mouse CD45.2 Antibody
- 产品名称：
- Alexa Fluor® 488 anti-mouse CD45.2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 109816
- 产品应用：
- 109816
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- B10.S mouse thymocytes and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.?
- Application References
(PubMed link indicates BioLegend citation) -
- Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
- Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
- Suzuki K, et al. 2000. Immunity 13:691. (IHC)
- Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
- Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
- Pascal V, et al. 2007. J. Immunol. 179:1751.
- Burman AC, et al. 2007. Blood 110:1064.
- Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
- Phan TG, et al. 2007. Nature Immunol. 8:992.
- Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
- Wen T, et al. 2013. PNAS. 110:6067. PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- Product Citations
-
- Enterina JR, et al. 2022. Cell Rep. 38:110512. PubMed
- Peterson EJ, et al. 2019. Mol Syst Biol. 15:e8584. PubMed
- Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
- ?zcan A, et al. 2020. J Invest Dermatol. 1003:140. PubMed
- Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
- Wang L, et al. 2020. Cells. 0.458333333. PubMed
- Wen T, et al. 2013. Proc Natl Acad Sci U S A. 110:6067. PubMed
- Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
- Jing Li et al. 2018. Immunity. 48(4):773-786 . PubMed
- Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
- Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
- Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed
- Tang Q, et al. 2021. Immunity. 54:1961. PubMed
- Inverso D, et al. 2021. Developmental Cell. 56(11):1677-1693.e10. PubMed
- Akkaya M, et al. 2020. Sci Rep. 10:13630. PubMed
- Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
- Hirasawa M, et al. 2016. J Biol Chem. 291: 7373-7385. PubMed
- Lever JM, et al. 2019. JCI Insight. 4:e125503. PubMed
- Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
- Zhang F, et al. 2019. Front Immunol. 2.077777778. PubMed
- Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
- RRID
- AB_492869 (BioLegend Cat. No. 109815) AB_492868 (BioLegend Cat. No. 109816)
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Suzuki K, et al. 2000. Immunity 13:691.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45.2 on UniProt.org
