Purified anti-Bcl-2 Antibody
- 产品名称：
- Purified anti-Bcl-2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 658701
- 产品应用：
- 658701
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified
IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 ?g per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 ?g/mL?is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- Pezzella F, et al. 1993. New Eng. J. Med. 329:690. (IHC)
- Pezzella F, et al. 1990. Am. J. Pathol. 137:225. (IHC)
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
- Okimoto T, et al. 2020. Cancer Sci. 111:1910. PubMed
- Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
- Kartika ID, et al. 2021. Cancer Med. 10:8988. PubMed
- Ga?án-Gómez I, et al. 2022. Nat Med. . PubMed
- Wang F, et al. 2020. Int J Oncol. 57:707. PubMed
- Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
- Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed
- NULL, et al. 2022. Cell. 185:916. PubMed
- Jiang Q, et al. 2022. Stem Cells Int. 2022:5897194. PubMed
- Zhou X, et al. 2019. Med Sci Monit. 25:836. PubMed
- Chong SJF, et al. 2020. Nucleic Acids Res. 48:12727. PubMed
- Wu YH, et al. 2020. Mol Med Rep. 21:851. PubMed
- Lu IT, et al. 2022. Mar Drugs. 20:. PubMed
- Inao T, et al. 2019. Cancer Sci. 110:2690. PubMed
- RRID
- AB_2562958 (BioLegend Cat. No. 658701) AB_2562959 (BioLegend Cat. No. 658702)
- Structure
- Two isoforms, 239 or 205 amino acids in length, with a predicted molecular weight of 26 kD or 22 kD and four ‘BH' domains.
- Distribution
-
Outer mitochondrial membrane, nuclear membrane, endoplasmic reticulum membrane.
- Function
- Regulates apoptosis through controlling mitochondrial fusion and fission.
- Interaction
- BAX, BAD, BAK, Bcl-X(L),EI24,APAF1, BBC3, BCL2L1, BNIPL, MRPL41, TP53BP2, FKBP8 BAG1, RAF1, EGLN3, G0S2, and BOP.
- Cell Type
- B cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Mitochondrial Function, Neuroscience, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
- Antigen References
-
1. Lin P, et al. 2013. Curr. Hematol. Malig. Rep. 8:243.
2. Tomita N. 2011. J. Clin. Exp. Hematop. 51:7.
3. Kelly PN, et al. 2011. Cell Death Differ. 18:1414.
4. Willimott S, et al. 2010. Biochem. Soc. Trans. 38:1571.
5. Rolland SG, et al. 2010. Curr. Opin. Cell Biol. 22:852.
- Gene ID
- 596 View all products for this Gene ID
- UniProt
- View information about Bcl-2 on UniProt.org
