PE anti-Bcl-2 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1980	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application References (PubMed link indicates BioLegend citation)

Product Citations

RRID

AB_2563281 (BioLegend Cat. No. 658707) AB_2563282 (BioLegend Cat. No. 658708)

Structure: Two isoforms, 239 or 205 amino acids in length, with a predicted molecular weight of 26 kD or 22 kD and four ‘BH' domains.
Distribution: Outer mitochondrial membrane, nuclear membrane, endoplasmic reticulum membrane.
Function: Regulates apoptosis through controlling mitochondrial fusion and fission.
Interaction: BAX, BAD, BAK, Bcl-X(L),EI24,APAF1, BBC3, BCL2L1, BNIPL, MRPL41, TP53BP2, FKBP8 BAG1, RAF1, EGLN3, G0S2, and BOP.
Cell Type: B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Mitochondrial Function, Neuroscience, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Antigen References: 1. Lin P, et al. 2013. Curr. Hematol. Malig. Rep. 8:243.
2. Tomita N. 2011. J. Clin. Exp. Hematop. 51:7.
3. Kelly PN, et al. 2011. Cell Death Differ. 18:1414.
4. Willimott S, et al. 2010. Biochem. Soc. Trans. 38:1571.
5. Rolland SG, et al. 2010. Curr. Opin. Cell Biol. 22:852.
Gene ID: 596 View all products for this Gene ID
UniProt: View information about Bcl-2 on UniProt.org