Alexa Fluor® 488 anti-Bcl-2 Antibody
- 产品名称：
- Alexa Fluor® 488 anti-Bcl-2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 658703
- 产品应用：
- 658703
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1908 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Pezzella F, et al. 1993. New Eng. J. Med. 329:690. (IHC)
- Pezzella F, et al. 1990. Am. J. Pathol. 137:225. (IHC)
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
- Nel I, et al. 2021. Diabetologia. 64(10):2306-2321. PubMed
- RRID
- AB_2563151 (BioLegend Cat. No. 658703) AB_2563152 (BioLegend Cat. No. 658704)
- Structure
- Two isoforms, 239 or 205 amino acids in length, with a predicted molecular weight of 26 kD or 22 kD and four ‘BH' domains.
- Distribution
-
Outer mitochondrial membrane, nuclear membrane, endoplasmic reticulum membrane.
- Function
- Regulates apoptosis through controlling mitochondrial fusion and fission.
- Interaction
- BAX, BAD, BAK, Bcl-X(L),EI24,APAF1, BBC3, BCL2L1, BNIPL, MRPL41, TP53BP2, FKBP8 BAG1, RAF1, EGLN3, G0S2, and BOP.
- Cell Type
- B cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Mitochondrial Function, Neuroscience, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
- Antigen References
-
1. Lin P, et al. 2013. Curr. Hematol. Malig. Rep. 8:243.
2. Tomita N. 2011. J. Clin. Exp. Hematop. 51:7.
3. Kelly PN, et al. 2011. Cell Death Differ. 18:1414.
4. Willimott S, et al. 2010. Biochem. Soc. Trans. 38:1571.
5. Rolland SG, et al. 2010. Curr. Opin. Cell Biol. 22:852.
- Gene ID
- 596 View all products for this Gene ID
- UniProt
- View information about Bcl-2 on UniProt.org
