APC/Fire™ 750 anti-human CD64 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1632	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

Baboon, Capuchin Monkey, Chimpanzee, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire? 750 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* APC/Fire? 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Application Notes

Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI^2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections¹².

Application References (PubMed link indicates BioLegend citation)

McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874.
Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Holl V, et al. 2004. J. Immunol. 173:6274.
Hober D, et al. 2002. J. Gen. Virol. 83:2169.
Cho HJ, et al. 2007. Physiol Genomics 149:60.
van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed
Bruhns P, et al. 2008. Blood 113:3716. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Carter DL, et al. 1999. Cytometry 37:41. (FC)
Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453.
Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)

RRID

AB_2650833 (BioLegend Cat. No. 305035) AB_2650834 (BioLegend Cat. No. 305036)

Antigen Details

Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, dendritic cells, activated granulocytes
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG receptor
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215.
Gene ID: 2209 View all products for this Gene ID
UniProt: View information about CD64 on UniProt.org

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