PE anti-mouse I-A-上海雅吉生物科技有限公司

规格	价格	库存
200µg	￥ 3876	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

C3H mouse splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include immunoprecipitation^1,2, protection against autoimmune IDDM³, in vitro blocking of antigen-specific MHC-restricted responses and in vivo inhibition of lymphoma growth⁴, and immunohistochemical staining⁵ of acetone-fixed frozen sections.

This clone does not cross react with other haplotypes (e.g. b, d, p, q).

Application References (PubMed link indicates BioLegend citation)

Landais D, et al. 1986. J. Immunol. 137:3002. (IP)
Kupinski JM, et al. 1983. J. Immunol. 130:2277. (IP)
Kappler JW, et al. 1981. J. Exp. Med. 153:1198.
Alisauskas RM, et al. 1986. Immunopharmacology 12:1.
Reis e Sousa and Germain 1999. J. Immunol. 162:6652. (IHC)
Yui MA, et al. 2010. J. Immunol. 185:284. PubMed
Gaudreau S, et al. 2007. J. Immunol. 179:3638. (FC)
Busman-Sahay K, et al. 2011. J. Immunol. 186:6710. PubMed.

Product Citations

Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
Wu J, et al. 2021. Mol Med Rep. 23:. PubMed
Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
Wang H, et al. 2020. Nat Mater. 1.655555556. PubMed
Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed

RRID

AB_313457 (BioLegend Cat. No. 109908)

Antigen Details

Structure: β chain of MHC class II
Distribution: B cell and activated T cells, APCs of H-2^b mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
Gene ID: 14960 View all products for this Gene ID
UniProt: View information about I-Ak on UniProt.org

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