内吞作用辅助蛋白EPN1抗体-上海雅吉生物科技有限公司

规格	价格	库存
50ul	￥ 1200	7
100ul	￥ 1900	6
200ul	￥ 2900	3

产品编号	Ys-13101R
英文名称	Epsin 1
中文名称	内吞作用辅助蛋白EPN1抗体
别名	EH domain binding mitotic phosphoprotein; EPN1; EPN 1; EPN-1; EPS 15 interacting protein 1; EPS-15-interacting protein 1; Epsin 1; EPN1_HUMAN.
研究领域	细胞生物神经生物学信号转导
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, (predicted: Mouse, Rat, Dog, Horse, Sheep, )
产品应用	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	60kDa
细胞定位	细胞核细胞浆细胞膜
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human Epsin 1/EPN1: 101-200/576
亚型	IgG
纯化方法	affinity purified by Protein A
缓冲液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	Epsin 1 is an endocytic accessory protein, with significant similarity to the Xenopus mitotic phosphoprotein MP90. Epsin 1 interacts with Eps15 (the ?subunit of the Clathrin adaptor AP2), Clathrin and other accessory proteins. The mitotic phosphorylation of these proteins may be one of the mechanisms by which the invagination of Clathrin-coated pits is blocked in mitosis. Both Epsin and Eps15, like other cytosolic components of the synaptic vesicle endo-cytic machinery, undergo constitutive phosphorylation and depolarization-dependent dephosphorylation in nerve terminals. Epsin 1 also contributes to the mechanism of Clathrin-vesicle-dependent endocytosis. The human Epsin 1 protein contains an Epsin N-terminal homology (ENTH) region and a single Clathrin-binding (LVDLD) motif. Epsin 1 localizes to the leading edge of a vesicular coated pit where the membrane is being actively bent. Function: Epsin 1 is a cytosolic adaptor protein and interacting partner for the EH domain containing region of Eps15. It is involved in the regulation of clathrin mediated endocytosis via its direct interactions with clathrin, the clathrin adaptor AP-2, and Eps15. Epsin 1 is a cargo specific adaptor for influenza virus entry through the clathrin mediated pathway. In addition to being a clathrin interacting protiein, Epsin 1 is also an ubiquitin, and phospholipid interacting protein. It also may function in a signaling pathway connecting the endocytic machinery to the regulation of nuclear function. Subunit: Monomer. Binds clathrin, ZBTB16/ZNF145 and ITSN1. Binds ubiquitinated proteins (By similarity). Binds REPS2, EPS15, AP2A1 and AP2A2. Interacts with RALBP1 in a complex also containing NUMB and TFAP2A during interphase and mitosis. Interacts with AP2B1. [SUBCELLULAR LOCATION] Subcellular Location: Cytoplasm (By similarity). Cell membrane; Peripheral membrane protein (By similarity). Nucleus (By similarity). Membrane, clathrin-coated pit (By similarity). Note=Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity). Post-translational modifications: Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation. Ubiquitinated (By similarity). Similarity: Belongs to the epsin family. Contains 1 ENTH (epsin N-terminal homology) domain. Contains 3 UIM (ubiquitin-interacting motif) repeats. SWISS:

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

联系我们