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The base medium for this cell line is OptiMEM 1 Reduced Serum Medium (Gibco Catalog No. 31985). To make the complete growth medium, add the following components to the base medium: 20 mM HEPES 10 mM GlutaMAX (Gibco Catalog No. 35050) 10 ng/mL Epidermal Growth Factor (EGF) fetal bovine serum (FBS) to a final concentration of 4%
