• Product Number: Ys-0437R

• English Name: Streptavidin

• Chinese Name: 链酶亲和素抗体

• Synonyms: SA protein; SA V1; SA V2; Streptavidin V1; Streptavidin V2; SAV1_STRVL

Antibody Details

• Source: Rabbit

• Clonality: Polyclonal

• Applications:

  • WB (Western Blot): 1:500-2000

  • ELISA: 1:5000-10000

  • IHC-F (Immunohistochemistry-Frozen): 1:100-500

  • IF (Immunofluorescence): 1:100-500 (antigen retrieval required for paraffin-embedded sections)

  • Not yet tested in other applications.

  • Optimal dilutions/concentrations should be determined by the end user.

• Theoretical Molecular Weight: 53 kDa

• Cellular Localization: Extracellular matrix; Secreted protein

• Form: Liquid

• Concentration: 1 mg/ml

• Immunogen: Streptavidin protein

• Isotype: IgG

• Purification Method: Affinity purified by Protein A

• Buffer: 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300, and 50% Glycerol

Storage and Handling

• Storage Conditions:

  • Shipped at 4°C.

  • Store at -20°C for one year.

  • Avoid repeated freeze/thaw cycles.

• Note: This product is intended for research use only, not for human, therapeutic, or diagnostic applications.

Product Description

Streptavidin is a biotin-binding protein originally isolated from Streptomyces avidinii. Unlike avidin, streptavidin is non-glycosylated and has a mildly acidic pI of 5. The recombinant form of streptavidin used in this product has a molecular weight of 53,000 daltons and a near-neutral pI. Streptavidin forms a tetrameric structure, with each subunit binding one molecule of biotin with high affinity, similar to avidin. It is more resistant to dissociation by guanidinium chloride compared to avidin.

Function

The biological function of streptavidin is not fully understood. It forms a strong, non-covalent, and specific complex with biotin (one biotin molecule per streptavidin subunit).

Subunit Structure

• Subunit: Homotetramer (By similarity).

Subcellular Localization

• Location: Secreted.

Sequence and Family

• Similarity: Belongs to the avidin/streptavidin family. Contains one avidin-like domain.

Database Information

• SWISS-PROT Accession: P22629

• Gene ID: N/A

Additional Notes

For further details, refer to the provided database links and product documentation.