SYBR Green miRNA荧光定量PCR试剂盒（文献引用产品）-上海雅吉生物科技有限公司

规格	价格	库存
100次	￥ 880	1

SYBR Green miRNA荧光定量PCR试剂盒应用Peter改良的miR-Q技术，采用特异性的上下游引物对反转录所得miRNA的cDNA产物进行定量检测^【1】。不同于其它公司的单条特异性引物扩增方法，HaiGene采用双向特异性扩增引物（原理如图1），从而极大提高了miRNA的检测特异性，其可以区分极高相似度的miRNA分子、减少非特异性扩增（图2，3所示）。该miRNA荧光定量PCR试剂盒对可以对任何miRNA分子进行检测。HG miRNA荧光定量PCR试剂盒共有一万余种，试剂盒编号为APXXXXX，每一个miRNA分子对应一个检测试剂盒，如果您研究的miRNA分子不在我们的列表中，请来信咨询，我们将及时给您优化设计。该试剂盒中的每一对引物均经过设计优化，确保扩增效率和特异性。使用该方法进行miRNA定量检测已被大量文献所采用^【2～11】。

组分

试剂盒组分名称	数量
2×Hi SYBR Green qPCR Mix	1 ml
50×ROX Reference Dye	200 μl
miRNA Primer F（10μM）	100 μl
miRNA Primer R（10μM）	100 μl
miRNA 标准品(1pM)	100 μl

储存

避光置于-20°C，可保存2年；避免反复冻融。

常用miRNA内参荧光定量PCR试剂盒

产品编号	产品名称	规格	价格(元)
AP01501	HG SYBR Green RNU6B miRNA荧光定量PCR试剂盒 (适用于组织和细胞样本）	100T×20μl	500
AP01511	HG SYBR Green miR-16 miRNA荧光定量PCR试剂盒（适用于全血样本）	100T×20μl	500

。文献参考：

文章标题：FAM30A Induces Inflammation and Oxidative Damage in PDLSCs by Targeting miR-424-5p

作者列表：Youlin Chang, Cheng Wang, Jing Zhang, Xiaodan Zhang, Yanqiang Gao, Ning Ma, Jianming Xie

影响因子：5.6

期刊：INTERNATIONAL DENTAL JOURNAL

发表时间：2026-5-15

DOI:10.1016/j.identj.2026.109605

文献主题：Abstract

Background: Glycine betaine (GB) not only plays an important role as an osmotic regulator in the regulation of abiotic stress in plants, but also serves as a methyl donor that affects the methylation level of the plant, which in turn affects its growth, development and response to adversity. Tomato as a major crop cultivated in facilities, facility soil salinization has become an important factor limiting efficient and high qualities tomato production. In contrast, little has been reported on the effect of GB on m6A methylation and its salt tolerance in tomato. In this study, the effects of exogenous GB on chlorophyll synthesis, photosynthesis and m6A methyltransferase in tomato under salt stress were investigated using tomato as experimental material.

Results: The results showed that 5 mM GB pretreatment alleviated the inhibitory effect of salt stress on the growth of tomato plants. GB promotes the synthesis and accumulation of Proto IX, Mg-Proto IX and protochlorophyllide in the chlorophyll synthesis pathway under salt stress by protecting the activities of CHLH (Magnesium-binding enzyme H subunit) and POR (protochlorophyllide oxidoreductase) thereby increasing the chlorophyll content. In addition, GB improves photosynthetic capacity of tomato by increasing stomatal opening and protecting Calvin cycle enzyme activity. And GB altered the expression level of m6A methyltransferase, which in turn indirectly affected the salt tolerance of tomato.

Conclusions: In summary, GB may enhance tomato salt tolerance by promoting chlorophyll synthesis, enhancing photosynthesis, and regulating the expression levels of m6A methyltransferase genes. These findings suggest that exogenous GB application could serve as an effective strategy to improve tomato productivity in salinized protected cultivation systems.

Keywords: Chlorophyll; Glycine betaine; Photosynthesis; Salt stress; m6A methyltransferase.