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His tag 标签抗体
产品名称:
His tag 标签抗体
英文名称:
His tag
产品类别:
抗体
产品编号:
Ys-0287R
产品应用:
WB=1:1000-5000
性状:
Liquid
纯化方法:
affinity purified by Protein A
保存条件:
-20℃
[价格]
规格 价格 库存
100ul ¥ 980 3
500ul ¥ 4000 1

产品详情

 产品类型:标签抗体 

研究领域:肿瘤  细胞生物  免疫学  染色质和核信号  信号转导  合成与降解  结合蛋白  
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Recombinant protein
产品应用:WB=1:1000-5000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性    状:Liquid
浓    度:1mg/ml
免 疫 原:Synthetic peptide: HHHHHH conjugated to KLH 
亚    型:IgG
纯化方法:affinity purified by Protein A
缓 冲 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
产品介绍:The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

SWISS:
N/A

Gene ID:
N/A

标签抗体
蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白
1) 6xHis与不同的Sepharose纯化填料结合(捆绑),可不受构象的影响,在天然或变形的条件下可进行进一步纯化。
2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
3) 6xHis标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括:细菌,杆状病毒和哺乳动物)
4) 6xHis标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能, 6xHis不会影响蛋白分泌
5) 6xHis标签免疫原性差,重组蛋白可以不必除去标签而直接作为抗原进行免疫

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