网尾线虫通用探针法荧光定量PCR试剂盒-上海雅吉生物科技有限公司

规格	价格	库存
50T	￥ 1836	200

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human BTLA transfected cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
?

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: inhibition of T cell proliferation and cytokine production¹. Clone MIH26 has agonistic activity on BTLA, resulting in the inhibition of activation.

The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for use in in vitro and in vivo biofunctional assays.

Application References (PubMed link indicates BioLegend citation)

Otsuki N, et al. 2006. Biochem. Bioph. Res. Co. 344:1121.
Okano M, et al. 2008. Clin. Exp. Allergy 38:1891.

Product Citations

Wei F, et al. 2013. Proc Natl Acad Sci U S A. 110:2480. PubMed
Xu X, et al. 2020. J Cell Biol. 219:00:00. PubMed
Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
Xu X, et al. 2021. Elife. 10:. PubMed
Ohue Y, et al. 2014. Clin Cancer Res. 20:5052. PubMed
Bziat V, et al. 2021. Cell. . PubMed
Zhang S, et al. 2021. Nat Commun. 12:2877. PubMed
Ye Z, et al. 2016. Sci Rep. 6:20401. PubMed

RRID

AB_2043945 (BioLegend Cat. No. 344505) AB_2065761 (BioLegend Cat. No. 344506)

Antigen Details

Structure: Ig superfamily, with similar structure to CTLA-4 and PD-1
Distribution: B cells and T lymphocytes, NK cells and dendritic cells
Function: Negative regulation of T cell activation, proliferation and cytokine production
Ligand/Receptor: HVEM
Cell Type: B cells, Dendritic cells, NK cells, T cells, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Watanabe N, et al. 2003. Nat. Immunol. 4:670.
2. Sun Y, et al. 2009. J. Immunol. 183:1946.
3. Gonzalez LC, et al. 2005. P. Natl. Acad. Sci. USA 102:1116.
Gene ID: 151888 View all products for this Gene ID
UniProt: View information about CD272 on UniProt.org

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