英文名称 CPA3
中文名称 肥大细胞羧肽酶A3抗体
别 名 carboxypeptidase A; Carboxypeptidase A3; carboxypeptidase A3 mast cell; CBPA3_HUMAN; Cpa3; Mast cell carboxypeptidase A; mast cell carboxypeptidase A3; MC CPA; MC-CPA.
研究领域 细胞生物 发育生物学 信号转导 干细胞
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Rat, (predicted: Human, Mouse, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 36kDa
细胞定位 细胞浆
性 状 Liquid
浓 度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from Human CPA3/MC-CPA:101-200/417
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Carboxypeptidase A (CPA) is a pancreatic exopeptidase which hydrolyses the peptide bond adjacent to the C-terminal end in polypeptide chains. Mast cell carboxypeptidase A (MC-CPA), a part of the peptidase M14 family, is a highly conserved metalloprotease localized to the secretory granules, along with trytases and chymases. MC-CPA is stored as an active enzyme in the granule and is released, along with other inflammatory mediators, upon mast cell degranulation. MC-CPA mirrors pancreatic carboxypeptidase A in cleaving COOH-terminal aromatic and aliphatic amino acid residues. The optimum pH of MC-CPA is between neutral and basic, depending upon the substrate. The MC-CPA gene, CPA3, resides on chromosome 3 and contains 11 exons.
Subcellular Location:
Cytoplasmic vesicle, secretory vesicle. Secretory granules.
Similarity:
Belongs to the peptidase M14 family.
SWISS:
P15088
Gene ID:
1359
Database links:
Entrez Gene: 1359 HumanOmim: 114851 HumanSwissProt: P15088 HumanUnigene: 646 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
| 产品图片 | Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CPA3) Polyclonal Antibody, Unconjugated (bs-12338R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
