上海细胞库
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
| 规格 | 价格 | 库存 |
|---|---|---|
| 50ul | ￥ 980 | 200 |
| 100ul | ￥ 1680 | 200 |
| 200ul | ￥ 2480 | 200 |
| 中文名称 | β3肾上腺素能受体抗体 |
| 别 名 | beta 3 Adrenergic Receptor; beta3-adrenergic receptor; beta3-AR; adrenergic; beta-3- receptor; ADR B3; ADRB 3; ADRB3R; Adrenergic beta 3 receptor; B3AR; Beta 3 adrenoceptor; Beta 3 adrenoreceptor; Beta 3AR; B3AR; BETA3AR; ADRB3_HUMAN. |
| 研究领域 | 心血管 细胞生物 神经生物学 生长因子和激素 细胞膜受体 内分泌病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Rat, (predicted: Mouse, Dog, Pig, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 44kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADRB3:310-408/408 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | beta 3 Adrenergic Receptor is located mainly in adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Beta adrenergic receptors are involved in the epenephrine and norepinephrine-induced activation of adenylate cyclase through the action of G proteins. beta 3 Adrenergic Receptor expression has been documented in adipose tissues, heart, and in smooth muscle of digestive and urinary tract organs (bladder, colon, small intestine, stomach, ureter). Utilization of alternate promoters and/or 3-prime untranslated regions may result in tissue-specific regulation of the expression of this protein. Function: Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed mainly in adipose tissues. Similarity: Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily. SWISS: P25962 Gene ID: 155 Database links: Entrez Gene: 155 Human Entrez Gene: 11556 Mouse Entrez Gene: 25645 Rat SwissProt: P13945 Human SwissProt: P25962 Mouse SwissProt: P26255 Rat Unigene: 2549 Human Unigene: 278475 Mouse Unigene: 10100 Rat Omim: 109691 HumanImportant Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ARβ3是新近发现的,继β1和β2之后的第3个β型肾上腺素受体亚型.分子量：44KDa(404AA) 近年来研究表明:β3-肾上腺素受体该受体基因突变或基因多态性与机体肥胖及心血管疾病相关;心力衰竭时该受体的含量明显增加,β3肾上腺素能受体与心血管疾病的相关研究可能为心血管疾病的预测、防治的新方法. |
| 产品图片 |  Sample:K562(Human) Cell Lysate at 40 ug Primary: Anti-beta 3 Adrenergic Receptor (bs-1063R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 54 kD  Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated(bs-1063R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: Hela.Primary Antibody (green line): Rabbit Anti-beta 3 Adrenergic Receptor antibody (bs-1063R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
