上海细胞库
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
| 规格 | 价格 | 库存 |
|---|---|---|
| 50ul | ￥ 980 | 200 |
| 100ul | ￥ 1680 | 200 |
| 200ul | ￥ 2480 | 200 |
| 中文名称 | 跨膜蛋白16A/钙激活氯离子通道抗体 |
| 别 名 | ANO 1;ANO1_HUMAN; Anoctamin 1; Anoctamin 1 calcium activated chloride channel; Anoctamin-1; Anoctamin1; Discovered on gastrointestinal stromal tumors protein 1; DOG 1; FLJ10261; Membrane protein; Oral cancer overexpressed 2; Oral cancer overexpressed protein 2; ORAOV 2; ORAOV2; TAOS 2; TAOS2; TMEM 16A; TMEM16A; Transmembrane protein 16A (eight membrane spanning domains); Transmembrane protein 16A; Tumor amplified and overexpressed sequence 2; Tumor-amplified and overexpressed sequence 2. |
| 研究领域 | 肿瘤 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 114kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DOG-1/TMEM16A:151-250/986 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | TMEM16A (Transmembrane protein 16A) is an eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products and is amplified in various cancers. It acts as a calcium-activated chloride channel and is required for normal tracheal development. Function: Acts as a calcium-activated chloride channel. Required for normal tracheal development (By similarity). Subcellular Location: Cell membrane; Multi-pass membrane protein. Cytoplasm. Tissue Specificity: Broadly expressed with higher levels in liver and skeletal muscle. Similarity: Belongs to the anoctamin family. SWISS: Q5XXA6 Gene ID: 55107 Database links: Entrez Gene: 55107 Human Entrez Gene: 101772 Mouse Entrez Gene: 309135 Rat Omim: 610108 Human SwissProt: Q5XXA6 Human SwissProt: Q8BHY3 Mouse Unigene: 503074 Human Unigene: 26700 Mouse Unigene: 162509 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:Lane 1: Skeletal muscle (Mouse) Lysate at 40 ug Lane 2: Skeletal muscle (Rat) Lysate at 40 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: A673 (Human) Cell Lysate at 30 ug Lane 5: U-2OS (Human) Cell Lysate at 30 ug Lane 6: MKN45 (Human) Cell Lysate at 30 ug Primary: Anti-ANO1/TMEM16A (bs-3794R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114-120 kD Observed band size: 120 kD  Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TMEM16A Polyclonal Antibody, Unconjugated(bs-3794R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TMEM16A Polyclonal Antibody, Unconjugated(bs-3794R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
