规格 | 价格 | 库存 |
---|---|---|
100ul | ¥ 1880 | 200 |
200ul | ¥ 2880 | 200 |
中文名称 | 钙结合蛋白D28K抗体 |
别 名 | CAB27; CALB 1; CALB; CALB1; Calbindin 1 28kDa; Calbindin-D-28K; Calbindin D28; D 28K; D28K; Vitamin D dependent calcium binding protein; Vitamin D dependent calcium binding protein avian type; Vitamin D dependent calcium binding protein avian-type; Vitamin D-dependent calcium-binding protein; CALB1_HUMAN. |
研究领域 | 细胞生物 免疫学 神经生物学 结合蛋白 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Rabbit, ) |
产品应用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 29kDa |
细胞定位 | 细胞浆 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Calbindin D28k:101-200/261 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
产品介绍 | Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. [provided by RefSeq, Jul 2008] Function: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Subunit: Interacts with RANBP9. Similarity: Belongs to the calbindin family. Contains 5 EF-hand domains. SWISS: P05937 Gene ID: 793 Database links: Entrez Gene: 793 Human Entrez Gene: 12307 Mouse Entrez Gene: 83839 Rat Omim: 114050 Human SwissProt: P05937 Human SwissProt: P12658 Mouse SwissProt: P07171 Rat Unigene: 65425 Human Unigene: 277665 Mouse Unigene: 3908 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 | ![]() Kidney(Rat) Lysate at 40 ug Primary: Anti-Calbindin (bs-3758R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 30kD ![]() Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Calbindin/Calbindin D28k Polyclonal Antibody, Unconjugated(bs-3758R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining ![]() Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Calbindin/Calbindin D28k Polyclonal Antibody, Unconjugated(bs-3758R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei |