上海细胞库
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
| 规格 | 价格 | 库存 |
|---|---|---|
| 50ul | ￥ 980 | 200 |
| 100ul | ￥ 1680 | 200 |
| 中文名称 | 多巴胺受体D1抗体 |
| 别 名 | DRD1; dopamine D1 receptor; D(1A) dopamine receptor; D1A dopamine receptor; Dopamine D1Receptors; D1DR; DADR; Dopamine Receptor D1; DR D1; DR D1A; DRD 1; DRD1 receptor; DRD1; DRD1A; DRD1_HUMAN; D(1A) dopamine receptor; DRD 1A; DRD1. |
| 研究领域 | 肿瘤 免疫学 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 50kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Dopamine Receptor D1:11-100/446 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008] Function: Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Subunit: Interacts with DNAJC14 via its C-terminus. Interacts with DRD1IP. Subcellular Location: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Tissue Specificity: Detected in caudate, nucleus accumbens and in the olfactory tubercle. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P21728 Gene ID: 1812 Database links: Entrez Gene: 1812 Human Entrez Gene: 13488 Mouse Entrez Gene: 24316 Rat Omim: 126449 Human SwissProt: P21728 Human SwissProt: Q61616 Mouse SwissProt: P18901 Rat Unigene: 2624 Human Unigene: 54161 Mouse Unigene: 24039 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:A549 Cell(Human)Lysate at 40 ugPrimary: Anti-Dopamine Receptor D1(bs-10610R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50kD Observed band size: 60kD  Sample:A431 Cell(Human)Lysate at 40 ug HepG2 Cell (Human) Lysate at 40 ug Primary: Anti-Dopamine Receptor D1(bs-10610R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50kD Observed band size: 60kD  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Dopamine Receptor D1 Polyclonal Antibody, Unconjugated(bs-10610R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Cell: SH-SY5YConcentration:1:100 Host/Isotype:Rabbit/IgG Flow cytometric analysis of Rabbit IgG isotype control (Cat#: bs-10610R) on SH-SY5Y(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody . |
