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FITM1蛋白抗体WB ELISA I
产品名称:
FITM1蛋白抗体WB ELISA I
英文名称:
FITM1
产品类别:
抗体
产品编号:
1609
产品应用:
WB ELISA IHC-P IHC-F ICC IF
性状:
Liquid
纯化方法:
affinity purified by Protein A
保存条件:
Shipped at 4℃. Store at -20 °C for one year. Avoid
[价格]
规格 价格 库存
100ul ¥ 1790 200
200ul ¥ 2680 200

产品详情

 

英文名称 FITM1
中文名称 FITM1蛋白抗体
别    名 Fat inducing protein 1; Fat inducing transcript 1; Fat storage inducing transmembrane protein 1; Fat storage-inducing transmembrane protein 1; Fat-inducing protein 1; FIT1; FITM 1; Fitm1; FITM1_HUMAN; MGC46490.  
研究领域 细胞生物  免疫学  信号转导  细胞类型标志物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Rat,  (predicted: Mouse, Dog, Cow, Horse, Rabbit, Sheep, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 32kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human FITM1:1-100/292 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 FIT1 belongs to an evolutionarily conserved family of proteins involved in fat storage (Kadereit et al., 2008 [PubMed 18160536]).[supplied by OMIM, May 2008]

Function:
Plays an important role in lipid droplet accumulation.

Subcellular Location:
Endoplasmic reticulum membrane.

Tissue Specificity:
Primarily expressed in heart and skeletal muscle.

Similarity:
Belongs to the FIT family.

SWISS:
A5D6W6

Gene ID:
161247

Database links:

Entrez Gene: 161247 Human

Entrez Gene: 68680 Mouse

Omim: 612028 Human

SwissProt: A7YWN2 Cow

SwissProt: A5D6W6 Human

SwissProt: Q91V79 Mouse

SwissProt: B2MVP8 Pig

Unigene: 128060 Human

Unigene: 36758 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Protein: 293T(human) lysate at 40ug;
Primary: rabbit Anti-FITM1 (bs-16090R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 32 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (Fat inducing transcript; FITM1) Polyclonal Antibody, Unconjugated (bs-16090R) at 1:400 overnight at 4∑C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (FITM1) Polyclonal Antibody, Unconjugated (bs-16090R) at 1:400 overnight at 4∑C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Fat inducing transcript; FITM1) Polyclonal Antibody, Unconjugated (bs-16090R) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

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