上海细胞库
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
| 规格 | 价格 | 库存 |
|---|---|---|
| 100ul | ￥ 1980 | 200 |
| 中文名称 | 磷酸化真核翻译起始因子4B抗体 |
| 别 名 | eIF4B (phospho S406); p-eIF4B (phospho S406); EIF 4B; EIF-4B; Eukaryotic initiation factor 4B; IF4B_HUMAN. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 转运蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 69kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human EIF4B around the phosphorylation site of Ser406:HP(p-S)WR |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot). Function: Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. Post-translational modifications: Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex. Similarity: Contains 1 RRM (RNA recognition motif) domain. SWISS: P23588 Gene ID: 1975 Database links: Entrez Gene: 1975 Human Entrez Gene: 75705 Mouse Entrez Gene: 300253 Rat Omim: 603928 Human SwissProt: P23588 Human SwissProt: Q8BGD9 Mouse Unigene: 648394 Human Unigene: 702041 Human Unigene: 290022 Mouse Unigene: 391795 Mouse Unigene: 95954 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Testis (Mouse) Lysate at 40 ugPrimary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD Sample: Testis (Mouse) Lysate at 40 ugPrimary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD  Sample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD Sample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD  Sample: Hela Cell (Human) Lysate at 40 ugPrimary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD Sample: Hela Cell (Human) Lysate at 40 ugPrimary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD  Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-EIF4B (Ser406)) Polyclonal Antibody, Unconjugated (bs-14551R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-EIF4B (Ser406)) Polyclonal Antibody, Unconjugated (bs-14551R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
