上海细胞库
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
| 规格 | 价格 | 库存 |
|---|---|---|
| 100ul | ￥ 1980 | 200 |
| 中文名称 | 磷酸化酪蛋白激酶1γ1+γ2+γ3抗体 |
| 别 名 | Casein kinase I isoforms gamma 1+2+3 (phospho Y263); p-CK1g2(phospho Y263); p-CSNK1G1(phospho Y263); p-CSNK1G2(phospho Y263); p-CSNK1G3(phospho Y263); Casein kinase 1 gamma 2; Casein kinase I isoform gamma 1; Casein kinase I isoform gamma 3; Casein kinase I isoform gamma-2; CK1g2; CKI gamma 1; CKI gamma 2; CKI gamma 3; CSNK1G1; CSNK1G2; CSNK1G3; KC1G1_HUMAN. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 49kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human Casein kinase I isoforms gamma 1+2+3 around the phosphorylation site of Tyr263:ER(p-Y)QK |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. They can phosphorylate a large number of proteins. Participates in Wnt signaling. Function: Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate (By similarity). Phosphorylates CLSPN. Subunit: Monomer. Subcellular Location: Cytoplasm. Post-translational modifications: Autophosphorylated (By similarity). Similarity: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. Contains 1 protein kinase domain. SWISS: Q9HCP0 Gene ID: 53944 Database links: Entrez Gene: 53944 Human Entrez Gene: 214897 Mouse Omim: 606274 Human SwissProt: Q9HCP0 Human SwissProt: Q8BTH8 Mouse Unigene: 646508 Human Unigene: 325816 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Brain (Mouse) Lysate at 40 ugPrimary: Anti-phospho-CSNK1G 1+2+3 ( Tyr263) (bs-12426R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD  Sample: Lymph node (Mouse) Lysate at 40 ugPrimary: Anti-phospho-CSNK1G 1+2+3 ( Tyr263) (bs-12426R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD  Sample: Bone (Mouse) Lysate at 40 ugPrimary: Anti-phospho-CSNK1G 1+2+3 ( Tyr263) (bs-12426R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD  Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CSNK1G 1+2+3 ( Tyr263)) Polyclonal Antibody, Unconjugated (bs-12426R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
