上海细胞库
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
| 规格 | 价格 | 库存 |
|---|---|---|
| 50ul | ￥ 1280 | 200 |
| 100ul | ￥ 1880 | 200 |
| 200ul | ￥ 2880 | 200 |
| 中文名称 | 肿瘤转移抑制蛋白BAMBI抗体 |
| 别 名 | BAMBI; BAMBI_HUMAN; BMP and activin membrane bound inhibitor homolog; BMP and activin membrane-bound inhibitor homolog; NMA; Non metastatic gene A protein; Non-metastatic gene A protein; Putative transmembrane protein NMA. |
| 研究领域 | 肿瘤 干细胞 生长因子和激素 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 27kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BAMBI:101-200/260 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | BAMBI is a membrane spanning glycoprotein that acts as a negative regulator of TGF-β signaling during development. The BAMBI family of proteins are related to type I TGF-β receptor family, however, BAMBI is a pseudoreceptor that lacks an intracellular serine/threonine kinase domain. BAMBI transcription regulation is under the influence of β-catenin, BMP, smad3 and smad4. BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues. BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines. Function: Negatively regulates TGF-beta signaling. Subcellular Location: Membrane. Tissue Specificity: High expression in kidney medulla, placenta and spleen; low in kidney cortex, liver, prostate and gut. Not expressed in normal skin, expression is high in melanocytes and in 3 out of 11 melanoma metastases tested. Similarity: Belongs to the BAMBI family. SWISS: Q13145 Gene ID: 25805 Database links: Entrez Gene: 25805 Human Entrez Gene: 68010 Mouse Entrez Gene: 449001 Pig Entrez Gene: 83837 Rat Omim: 604444 Human SwissProt: Q13145 Human SwissProt: Q9D0L6 Mouse SwissProt: Q91XN4 Rat Unigene: 533336 Human Unigene: 284863 Mouse Unigene: 25267 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:Lane 1: K562 (Human) Cell Lysate at 30 ug Lane 2: MCF-7 (Human) Cell Lysate at 30 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: A549 (Human) Cell Lysate at 30 ug Primary: Anti-BAMBI (bs-12418R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 30 kD  Sample:RAW264.7 (Mouse) CellLysate at 30 ug Primary: Anti-BAMBI (bs-12418R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 30 kD  Sample:Testis (Mouse) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug Primary: Anti-BAMBI (bs-12418R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 30 kD  Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BAMBI Polyclonal Antibody, Unconjugated(bs-12418R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
