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维甲酸诱导蛋白17抗体
产品名称:
维甲酸诱导蛋白17抗体
英文名称:
Retinoic acid induced 17
产品类别:
抗体
产品编号:
7709
产品应用:
WB ELISA IHC-P IHC-F Flow-Cyt IF
性状:
Liquid
纯化方法:
affinity purified by Protein A
保存条件:
Shipped at 4℃. Store at -20 °C for one year. Avoid
[价格]
规格 价格 库存
100ul ¥ 1680 200
200ul ¥ 2480 200

产品详情

 

中文名称 维甲酸诱导蛋白17抗体
别    名 Zinc finger MIZ domain-containing protein 1; MIZ; PIAS like protein hZimp10; PIAS like protein on chromosome 10; PIAS like protein Zimp10; PIAS-like protein Zimp10; RAI 17; RAI17; RAI-17; Retinoic acid induced 17; Retinoic acid induced protein 17; Retinoic acid-induced protein 17; TRAFIP10; Zimp10; Zinc finger containing Miz1 PIAS like protein on chromosome 10; Zinc finger MIZ domain-containing protein 1; Zinc finger MIZ-type containing 1; Zmiz1; ZMIZ1_HUMAN; hZIMP10; KIAA1224.  
研究领域 细胞生物  细胞周期蛋白  转录调节因子  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Sheep, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 115kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Retinoic acid induced 17:51-150/1067 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation.
Tissue specificity:Expressed most abundantly in ovary and, at lower levels, in prostate, spleen and testis. Weak expression, if any, in thymus, small intestine, colon and peripheral blood leukocytes.

Function:
Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation.

Subunit:
Interacts with AR, but not with ESR1, NR3C1, PGR, THRB nor VDR.

Subcellular Location:
Nucleus speckle. Cytoplasm.

Tissue Specificity:
Expressed most abundantly in ovary and, at lower levels, in prostate, spleen and testis. Weak expression, if any, in thymus, small intestine, colon and peripheral blood leukocytes.

Similarity:
Contains 1 SP-RING-type zinc finger.

SWISS:
Q9ULJ6

Gene ID:
57178

Database links:
UniProtKB/Swiss-Prot: Q9ULJ6.3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Retinoic) Polyclonal Antibody, Unconjugated (bs-7709R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Retinoic) Polyclonal Antibody, Unconjugated (bs-7709R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Retinoic) Polyclonal Antibody, Unconjugated (bs-7709R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Retinoic acid induced 17 Antibody(bs-7709R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

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