上海细胞库
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
| 规格 | 价格 | 库存 |
|---|---|---|
| 100ul | ￥ 1980 | 200 |
Entrez Gene: 477753 Dog Entrez Gene: 240 Human Entrez Gene: 11689 Mouse Entrez Gene: 25290 Rat Omim: 152390 Human SwissProt: P09917 Human SwissProt: P48999 Mouse SwissProt: P12527 Rat Unigene: 89499 Human Unigene: 41072 Mouse Unigene: 9662 Rat
中文名称 磷酸化5-脂氧合酶抗体 别 名 5-Lipoxygenase (Phospho Ser663); 5-Lipoxygenase (Phospho S663); 5-lipoxygenase; 5 LO; 5LPG; ALOX 5; ALOX5; Arachidonate 5 lipoxygenase; Arachidonic acid 5 lipoxygenase; Leukotriene A4 synthase; LOG 5; LOG5; MGC163204; LOX5_HUMAN. 产品类型 磷酸化抗体 研究领域 肿瘤 免疫学 信号转导 转录调节因子 激酶和磷酸酶 合成与降解 抗体来源 Rabbit 克隆类型 Polyclonal 交叉反应 Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Rabbit, ) 产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user. 分 子 量 78kDa 细胞定位 细胞核 细胞浆 细胞膜 性 状 Liquid 浓 度 1mg/ml 免 疫 原 KLH conjugated synthesised phosphopeptide derived from rat 5-Lipoxygenase around the phosphorylation site of Ser663:YL(p-S)PD 亚 型 IgG 纯化方法 affinity purified by Protein A 储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. PubMed PubMed 产品介绍 This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.
Function:
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Subunit:
Interacts with ALOX5AP and LTC4S.
Subcellular Location:
Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein.
Post-translational modifications:
Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
Similarity:
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain.
SWISS:
P09917
Gene ID:
25290
Database links:
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
5-脂氧合酶蛋白(5-LOX; 5-lipoxygenase; Arachidonate 5-lipoxygenase; 5-LO; ALOX5 )是花生四烯酸代谢途径中的一种关键酶,在恶性肿瘤的发生、发展过程中起了重要作用.抑制5-脂氧合酶及其产物的表达有可能预防和逆转恶性肿瘤的发生。 产品图片 
Sample:
Heart (Rat) Lysate at 40 ug
Primary: Anti- Phospho-5-Lipoxygenase (Ser663) (bs-3252R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 75 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-5-Lipoxygenase (Ser663)) Polyclonal Antibody, Unconjugated (bs-3252R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-5-Lipoxygenase (Ser663)) Polyclonal Antibody, Unconjugated (bs-3252R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-5-Lipoxygenase(Ser663) Polyclonal Antibody, Unconjugated(bs-3252R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
