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P物质抗体
产品名称:
P物质抗体
英文名称:
ubstance P
产品类别:
抗体
产品编号:
0065R
产品应用:
ELISA IHC-P IHC-F Flow-Cyt IF
性状:
Liquid
纯化方法:
affinity purified by Protein A
保质期:
1年
保存条件:
Shipped at 4℃. Store at -20 °C for one year. Avoid
[价格]
规格 价格 库存
50ul ¥ 1200 14
100ul ¥ 1900 8
200ul ¥ 2900 4

产品详情

 

中文名称 P物质抗体
别    名 Hs.2563; Neurokinin 1; Neurokinin 2; Neurokinin A; Neurokinin alpha; Neuromedin L; Neuropeptide gamma; NKA; NKNA; PPT; Protachykinin 1 precursor; Substance K; Substance P; TAC1; TAC2; Tachykinin 1; Tachykinin 2; Tachykinin precursor 1; Tachykinin1; TACI; TKN1_HUMAN.  

 

研究领域 神经生物学  新陈代谢  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Mouse, Rat,  (predicted: Human, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig, )
产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 1.4/13kDa
细胞定位 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Substance P(RPKPQQFFGLM):58-68/129 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.

Subcellular Location:
Secreted.

Similarity:
Belongs to the tachykinin family.

SWISS:
P20366

Gene ID:
6863

Database links:

Entrez Gene: 6863 Human

Entrez Gene: 21333 Mouse

Entrez Gene: 24806 Rat

Omim: 162320 Human

SwissProt: P20366 Human

SwissProt: P41539 Mouse

SwissProt: P06767 Rat

Unigene: 2563 Human

UniProtKB/Swiss-Prot: P20366.1



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

P物质(Substance P)是脑组织及消化道中发现的一种神经递质肽。由130个氨基酸残基组成,胃肠道中肌肉收缩的有力促进剂,亦为强血管扩张剂。
SP是一种脑肠肽,亦是最早发现的一种神经肽,属于速激肽家族成员,它广泛分布于中枢及外周神经系统,作为一种神经递质或调质参与疼痛、免疫、心血管等多种生理功能的调节。SP与其它的递质或调质类似,能作用于多种类型的受体,速激肽家族有NK1、NK2、NK3三种受体,都能与SP结合,其中NK1与SP的结合能力最强而被称为SP受体。
产品图片

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ( Substance P) Polyclonal Antibody, Unconjugated (0065R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control: Mouse brain cells (blue).
Primary Antibody:Rabbit Anti- Substance P antibody(bs-0065R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (0065R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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