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钠-糖共转运载体1抗体
产品名称:
钠-糖共转运载体1抗体
英文名称:
SGLT1
产品类别:
抗体
产品编号:
1128
产品应用:
WB ELISA IHC-P IHC-F IF
性状:
Liquid
纯化方法:
affinity purified by Protein A
保质期:
1年
保存条件:
Shipped at 4℃. Store at -20 °C for one year. Avoid
[价格]
规格 价格 库存
50ul ¥ 980 200
100ul ¥ 1680 200
200ul ¥ 2480 200

产品详情

 

中文名称 钠-糖共转运载体1抗体
别    名 D22S675; High affinity sodium glucose cotransporter 1; GLT1; GLT-1; High affinity sodium glucose cotransporter; Human Na+/glucose cotransporter 1; Na(+)/glucose cotransporter 1; NAGT; SGLT 1; SLC5A1; Sodium glucose cotransporter 1; Sodium/glucose cotransporter 1; Solute carrier family 5 (sodium/glucose cotransporter) member 1; Solute carrier family 5 member 1. 

 

 
研究领域 神经生物学  转运蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Rat,  (predicted: Mouse, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 73kDa
细胞定位 细胞外基质 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SGLT1:401-500/665 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Function:
Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed mainly in intestine and kidney.

Post-translational modifications:
N-glycosylation is not necessary for the cotransporter function.

DISEASE:
Congenital glucose/galactose malabsorption (GGM) [MIM:606824]: Intestinal monosaccharide transporter deficiency. It is an autosomal recessive disorder manifesting itself within the first weeks of life. It is characterized by severe diarrhea and dehydration which are usually fatal unless glucose and galactose are eliminated from the diet. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

SWISS:
Q8C3K6

Gene ID:
6523

Database links:

Entrez Gene: 6523 Human

Entrez Gene: 20537 Mouse

Entrez Gene: 397113 Pig

Entrez Gene: 25552 Rat

Omim: 182380 Human

SwissProt: P13866 Human

SwissProt: Q8C3K6 Mouse

SwissProt: P53790 Rat

Unigene: 1964 Human

Unigene: 25237 Mouse

Unigene: 10224 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

葡萄糖的跨膜转运主要是通过SGLT1结合1 mol葡萄糖,2 mol的Na+,形成Na+-载体-葡萄糖复合物,顺Na+的浓度梯度进入细胞.不同物种的SGLT1具有较高的同源性.
产品图片 Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-SGLT1 (bs-1128R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kD
Observed band size: 60 kD
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SGLT1) Polyclonal Antibody, Unconjugated (bs-1128R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min Block endogenous peroxidase by 3% Hydrogen peroxide for 30min Blocking buffer (normal goat serum) at 37℃ for 20 min Incubation: Anti-SGLT1 Polyclonal Antibody, Unconjugated(bs-1128R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

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