产品分类/PRODUCT CLASSIFICATION
| 规格 | 价格 | 库存 |
|---|---|---|
| 50ul | ￥ 980 | 200 |
| 100ul | ￥ 1980 | 200 |
| 200ul | ￥ 2480 | 200 |
| 中文名称 | 细胞核受体Rev-Erbα抗体 |
| 别 名 | EAR-1; EAR1; ERBA-RELATED 1; hRev; Nr1d1; NR1D1_HUMAN; Nuclear Receptor Rev-ErbA Alpha; Nuclear receptor subfamily 1 group D member 1; Orphan nuclear receptor NR1D1; Rev erbAalpha; Rev erbalpha; Rev-erbA-alpha; Rev-ErbAalpha; REV-ERBalpha; THRA1; THRAL; Thyroid hormone receptor alpha-like; Thyroid hormone receptor, alpha like; V-erbA related protein EAR-1; V-erbA-related protein 1. |
| 研究领域 | 细胞生物 免疫学 染色质和核信号 神经生物学 信号转导 生长因子和激素 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Cow, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 67kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NR1D1:551-614/614 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | NR1D1, a NR1 Thyroid Hormone-Like Receptor, is encoded by the same genomic locus as, but transcribed from the opposite strand of, Thyroid Hormone Receptor Alpha (TR Alpha). NR1D1 is a target of Nuclear Receptor ROR Alpha and a transcription regulator that has been shown to affect myocyte differentiation, adipogenesis, and lipoprotein metabolism. Mice lacking NR1D1 show abnormal postnatal cerebellar development. NR1D1 expression has been documented in human skeletal muscle and a variety of mouse and rat tissues. ESTs have been isolated from human tissue libraries, including cancerous adrenal, blood, brain, breast, colon, duodenum, fetus, head/neck, kidney, lung, skeletal muscle, skin, synovium, uterus, normal brain, breast, colon, eye, heart, pancreas, pituitary, prostate, skeletal muscle, skin, testis and thyroid. Function: Functions as a constitutive transcriptional repressor. In collaboration with SP1, activates GJA1 transcription. Possible receptor for triiodothyronine. Subunit: Interacts with C1D and NR2E3. Interacts with SP1. Subcellular Location: Nucleus (Potential). Tissue Specificity: Expressed in all tissues and cell lines examined. Expressed at high levels in some squamous carcinoma cell lines. Similarity: Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: P20393 Gene ID: 9572 Database links: Entrez Gene: 9572 Human Entrez Gene: 768225 Cow Entrez Gene: 217166 Mouse Entrez Gene: 252917 Rat Omim: 602408 Human SwissProt: Q08E02 Cow SwissProt: P20393 Human SwissProt: Q3UV55 Mouse SwissProt: Q63503 Rat Unigene: 592130 Human Unigene: 724 Human Unigene: 390397 Mouse Unigene: 29848 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:BRL-3A (Rat) Cell Lysate at 40 ug Primary: Anti- NR1D1 (bs-3563R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 67 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NR1D1) Polyclonal Antibody, Unconjugated (bs-3563R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Tissue/cell: human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NR1D1 Polyclonal Antibody, Unconjugated(bs-3563R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NR1D1/REV-ERB alpha Polyclonal Antibody, Unconjugated(bs-3563R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
