产品分类/PRODUCT CLASSIFICATION
| 规格 | 价格 | 库存 |
|---|---|---|
| 100ul | ￥ 1980 | 200 |
| 200ul | ￥ 2980 | 200 |
| 中文名称 | 孤菲肽受体/痛敏肽受体抗体 |
| 别 名 | H.sapiens mRNA for ORL1 receptor; Kappa type 3 opioid receptor; Kappa-type 3 opioid receptor; KOR 3; KOR-3; KOR3; LC132 receptor like; MGC34578; Nociceptin receptor; NOCIR; OOR; Opiate receptor like 1; Opiod receptor like 1; Oprl1; OPRX_HUMAN; ORL1; Orphanin FQ receptor. |
| 研究领域 | 细胞生物 神经生物学 信号转导 细胞凋亡 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Guinea Pig, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 40kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nociceptin receptor:1-47/370 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Nociceptin receptor Receptor for the neuropeptide nocipeptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase[subcellular location] integral membrane protein. Belongs to the G-protein coupled receptor 1 family. Function: Receptor for the neuropeptide nociceptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: In the brain, isoform KOR3 and isoform KOR3C are most abundant in hypothalamus and periaqueductal gray. Isoform KOR3A is highly expressed in cortex, striatum and brainstem. Isoform KOR3D is highly expressed in cerebellum, hypothalamus and brainstem. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P35377 Gene ID: 4987 Database links: Entrez Gene: 4987 Human Entrez Gene: 18389 Mouse Entrez Gene: 29256 Rat Omim: 602548 Human SwissProt: P41146 Human SwissProt: P35377 Mouse SwissProt: P35370 Rat Unigene: 2859 Human Unigene: 285075 Mouse Unigene: 9762 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | Sample: Heart(Rat) lysate at 30ug; Brain(Rat) lysate at 30ug; Primary: Anti-Nociceptin receptor (bs-0181R) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 40kD Observed band size : 40kD  Sample:Heart (Mouse) Lysate at 40 ug Primary: Anti- Nociceptin receptor (bs-0181R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 52 kD 免疫组化（IHC）Immunohistochemistry Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated(bs-0181R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  免疫荧光Immunofluorescence 组织切片： Tissue/cell: human placenta tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated(bs-0181R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. |
