规格 | 价格 | 库存 |
---|---|---|
50ul | ¥ 980 | 200 |
100ul | ¥ 1680 | 200 |
200ul | ¥ 2480 | 200 |
中文名称 | 白介素9抗体 |
别 名 | Interleukin-9; IL9; Homolog of mouse T cell and mast cell growth factor 40; HP40; IL 9; Mast cell growth factor; MCGF; Megakaryoblast growth factor; p40 cytokine; p40 T cell and mast cell growth factor; p40 T-cell and mast cell growth factor; T cell growth factor 3; T cell growth factor p40; T-cell growth factor p40; Interleukin 9; TCGF 3. |
研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 信号转导 细胞凋亡 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Mouse, (predicted: Rat, ) |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:200-800 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 16kDa |
细胞定位 | 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from mouse IL-9:21-120/144 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
产品介绍 | The protein encoded by this gene is a cytokine that actsas a regulator of a variety of hematopoietic cells. This cytokinestimulates cell proliferation and prevents apoptosis. It functionsthrough the interleukin 9 receptor (IL9R), which activatesdifferent signal transducer and activator (STAT) proteins and thusconnects this cytokine to various biological processes. The geneencoding this cytokine has been identified as a candidate gene forasthma. Genetic studies on a mouse model of asthma demonstratedthat this cytokine is a determining factor in the pathogenesis ofbronchial hyperresponsiveness. [provided by RefSeq, Jul 2008]. Function: Supports IL-2 independent and IL-4 independent growth of helper T-cells. Subcellular Location: Secreted. Similarity: Belongs to the IL-7/IL-9 family. SWISS: P15247 Gene ID: 16198 Database links: Entrez Gene: 3578 Human Entrez Gene: 16198 Mouse Omim: 146931 Human SwissProt: P15248 Human SwissProt: P15247 Mouse Unigene: 960 Human Unigene: 3006 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 白介素-9是由CD4和辅助性T细胞的Th2细胞分泌的细胞因子,具有多种生物学活性,在机体的免疫应答中具有多种免疫调节作用,IL-9参与肿瘤发生、寄生虫免疫、支气管哮喘、慢性阻塞性肺病和器官移植等多种疾病过程。 |
产品图片 | Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-IL-9 (bs-2428R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 16 kD Observed band size: 16 kD Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated (bs-2428R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-9 Polyclonal Antibody, Unconjugated(bs-2428R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Mouse mammary tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated (bs-2428R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining |