产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 血管紧张素II受体样蛋白1抗体 |
| 别 名 | APJ receptor; AGTR L1; AGTRL 1; AGTRL1; Angiotensin II receptor like 1; Angiotensin receptor like 1; Apelin receptor; APJ; B78; FLJ90771; G protein coupled receptor APJ; GPCR34; HG 11; HG11; MGC45246; MSR; msr/apj; Aplnr; Agtrl1; APLNR; FLJ96609; APJ_HUMAN. |
| 研究领域 | 心血管 细胞生物 免疫学 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Chicken, Dog, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 42kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Apelin Receptor :181-280/380 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | This gene encodes a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified. [provided by RefSeq Function: Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia. Subcellular Location: Cell membrane. Tissue Specificity: Widely expressed in the brain, in glial cells, astrocytes and neuronal subpopulations, as well as in the spleen, thymus, ovary, small intestine and colon. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P35414 Gene ID: 187 Database links: Entrez Gene: 429337 Chicken Entrez Gene: 615435 Cow Entrez Gene: 187 Human Entrez Gene: 23796 Mouse Entrez Gene: 83518 Rat Omim: 600052 Human SwissProt: P35414 Human SwissProt: Q9WV08 Mouse SwissProt: Q9JHG3 Rat Unigene: 438311 Human Unigene: 29368 Mouse Unigene: 7965 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:Lane 1: K562 (Human) cell Lysate at 30 ug Primary: Anti-Apelin Receptor (bs-21310R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD  Sample:Lane 1: Lung (Rat) Lysate at 40 ug Lane 2: Spleen (Rat) Lysate at 40 ug Primary: Anti-Apelin Receptor (bs-21310R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD  Sample:Lane 1: Spleen (Mouse) Lysate at 40 ug Lane 2: Lung (Mouse) Lysate at 40 ug Lane 3: Thymus (Mouse) Lysate at 40 ug Primary: Anti-Apelin Receptor (bs-21310R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Apelin Receptor) Polyclonal Antibody, Unconjugated (bs-21310R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
