产品分类/PRODUCT CLASSIFICATION
| 中文名称 | CD20抗体 |
| 别 名 | CD20_HUMAN; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; MS4A1; MS4A2; B1; CVID5; LEU-16; S7. |
| 研究领域 | 肿瘤 细胞生物 免疫学 干细胞 细胞类型标志物 淋巴细胞 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg /Test not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 33kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD20:101-200/297 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit. Function: This protein may be involved in the regulation of B-cell activation and proliferation Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Expressed on B-cells. Post-translational modifications: Phosphorylated. Might be functionally regulated by protein kinase(s). DISEASE: Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Similarity: Belongs to the MS4A family. SWISS: P11836 Gene ID: 931 Database links: Entrez Gene: 931 Human Omim: 112210 Human SwissProt: P11836 Human Unigene: 712553 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. B淋巴细胞标志物 CD20是B细胞特异性抗原，其表达呈细胞类型一种质膜蛋白质，参与信号传导作用，此抗体识别大多数B细胞上的一种抗原。多用于标记B淋巴细胞及B细胞淋巴瘤。 CD20一般不与T细胞交叉反应，对B细胞淋巴瘤和急慢性淋巴细胞白血病等有较好的细胞特异性，因此在恶性淋巴瘤，尤其是T或B细胞淋巴瘤的分类上，CD20是最常用的B细胞标志。 |
| 产品图片 |  Sample:Lane 1: Large intestine (Mouse) Lysate at 40 ug Lane 2: Bone (Mouse) Lysate at 40 ug Primary: Anti-CD20 (bs-20638R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD  Sample:Raji(Human) Cell Lysate at 30 ug Primary: Anti-CD20 (bs-20638R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD  Sample:Lymph node (Mouse) Lysate at 40 ug Primary: Anti-CD20 (bs-20638R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD  Sample:Spleen (Mouse)Lysate at 40 ug Lymph node(Mouse)Lysate at 40 ug Primary: Anti-CD20 (bs-20638R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 33kD Observed band size: 35kD  Blank control(mouse): Mouse Spleen(fixed with 2% paraformaldehyde for 10 min at 37℃).Primary Antibody:Rabbit Anti-CD20 antibody (bs-20638R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
