产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 磷酸化p21激活激酶1抗体 |
| 别 名 | PAK1 (phospho S204); p-PAK1 (phospho S204); ADRB2; Alpha PAK; Alpha-PAK; MGC130000; MGC130001; p21 activated kinase 1; p21 protein (Cdc42/Rac) activated kinase 1; p21-activated kinase 1; p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related); p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p65 PAK; p65-PAK; P68-PAK; PAK alpha; PAK-1; Pak1; PAK1_HUMAN; Paka; PAKalpha; Protein kinase MUK2; Rac/p21-activated kinase; Serine/threonine-protein kinase PAK 1; STE20 homolog yeast. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞凋亡 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 61kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human PAK1 around the phosphorylation site of Ser204:TR(p-S)VI |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010] Function: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Subcellular Location: Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation. Post-translational modifications: Autophosphorylated when activated by CDC42/p21 and RAC1. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 protein kinase domain. SWISS: Q13153 Gene ID: 5058 Database links: Entrez Gene: 5058 Human Entrez Gene: 18479 Mouse Entrez Gene: 29431 Rat Omim: 602590 Human SwissProt: Q13153 Human SwissProt: O88643 Mouse SwissProt: P35465 Rat Unigene: 435714 Human Unigene: 260227 Mouse Unigene: 9149 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 2: NIH/3T3(Mouse) Cell Lysate at 30 ug Lane 3: Pancreas (Rat) Lysate at 40 ug Lane 4: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-phospho-PAK1 (Ser204) (bs-4318R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75 kD Observed band size: 75 kD |
