产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 蛋白磷酸酶3调节亚基C抗体 |
| 别 名 | calcineurin A gamma; CALCINEURIN A3; Calcineurin; Calcineurin testis specific catalytic subunit; Calmodulin dependent calcineurin A subunit gamma isoform; Calmodulin-dependent calcineurin A subunit gamma isoform; CALNA3; CAM PRP catalytic subunit; CAM-PRP catalytic subunit; CNA3; pp2b gamma; ; PP2BC_HUMAN; PP2Bgamma; ; PPP3CC; protein phosphatase 3 (formerly 2B) catalytic subunit gamma isoform (calcineurin A gamma); protein phosphatase 3 (formerly 2B) catalytic subunit gamma isoform; Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform; testis-specific catalytic subunit. |
| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 58kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PPP3CC:51-150/512 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Calcineurin is a calcium-dependent, calmodulin-stimulated protein phosphatase involved in the downstream regulation of dopaminergic signal transduction. Calcineurin is composed of a regulatory subunit and a catalytic subunit. The protein encoded by this gene represents one of the regulatory subunits that has been found for calcineurin. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] Function: Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Subunit: Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity. Tissue Specificity: Testis. Similarity: Belongs to the PPP phosphatase family. PP-2B subfamily. SWISS: P48454 Gene ID: 5533 Database links: Entrez Gene: 5533 Human Entrez Gene: 19057 Mouse Entrez Gene: 171378 Rat Omim: 114107 Human SwissProt: P48454 Human SwissProt: P48455 Mouse Unigene: 728861 Human Unigene: 731683 Human Unigene: 439683 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Testis(Mouse)Lysate at 40 ugPrimary: Anti-PPP3CC(bs-19973R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 58kD Observed band size: 58kD  Sample: A549 (Human)Cell Lysate at 40 ugPrimary: Anti-PPP3CC(bs-19973R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 58kD Observed band size: 58kD  Sample:Hela(Human) Cell Lysate at 40 ug Primary: Anti-PPP3CC (bs-19973R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD  Sample:DU145(Human) Cell Lysate at 40 ug Primary: Anti-PPP3CC (bs-19973R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD  Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP3CC) Polyclonal Antibody, Unconjugated (bs-19973R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. |
