产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 肌动蛋白相关蛋白2/3复合物亚基3抗体 |
| 别 名 | Actin related protein 2/3 complex subunit 3; ARC21; ARPC3_HUMAN; ARP2/3 complex 21 kDa subunit; ARP2/3 protein complex subunit p21; ARPC3. |
| 研究领域 | 细胞生物 信号转导 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Xenopus laevis (predicted: Rat, Chicken, Cow, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 20kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human p21-ARC:2-100/178 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been conserved through evolution and is implicated in the control of actin polymerization in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013] Function: p21-ARC is part of a complex implicated in the control of actin polymerization in cells. It belongs to a complex composed of ARP2, ARP3, ARPC1, ARPC2, ARPC3, ARPC4 and ARPC5, collectively known as the ARPC3 family. p21-ARC is implicated as an important regulator of growth cone translocation, especially in the CNS (Strasser et al, 2004). Subunit: Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and Subcellular Location: Cytoplasm, cytoskeleton. Cell projection. Similarity: Belongs to the ARPC3 family. SWISS: O15145 Gene ID: 10094 Database links: Entrez Gene: 10094 Human Entrez Gene: 56378 Mouse Entrez Gene: 288669 Rat Entrez Gene: 415204 Zebrafish Omim: 604225 Human SwissProt: O15145 Human SwissProt: Q9JM76 Mouse Unigene: 524741 Human Unigene: 275942 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Protein: intestinal(mouse) lysate at 30ug;Primary: rabbit Anti-p21-ARC (bs-17589R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:20 kD Observed band size: 20 kD  Protein:intestinal(mouse) lysate at 30ug; Raji(human) lysate at 30ug; Primary: rabbit Anti-p21-ARC (bs-17589R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:20 kD Observed band size: 20 kD  Sample:Hela(Human) Cell Lysate at 30 ug Primary: Anti- p21-ARC (bs-17589R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 20 kD  Sample:Spleen (Mouse) Lysate at 40 ug Primary: Anti-p21-ARC (bs-17589R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 20 kD  Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p21-ARC) Polyclonal Antibody, Unconjugated (bs-17589R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
