产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 5-羟色胺受体2A抗体 |
| 别 名 | SR-2A; 5 HT 2; 5 HT 2A; 5 HT2 receptor; 5 HT2A; 5 hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; 5-hydroxytryptamine 2A receptor; 5-hydroxytryptamine receptor 2A; 5HT2A_HUMAN; HTR 2; HTR 2A; HTR2; HTR2A; Serotonin receptor 2A. |
| 研究领域 | 心血管 细胞生物 神经生物学 细胞膜受体 G蛋白偶联受体 新陈代谢 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 53kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human 5HT2A Receptor:51-150/471 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | This gene encodes one of the receptors for serotonin, a neurotransmitter with many roles. Mutations in this gene are associated with susceptibility to schizophrenia and obsessive-compulsive disorder, and are also associated with response to the antidepressant citalopram in patients with major depressive disorder (MDD). MDD patients who also have a mutation in intron 2 of this gene show a significantly reduced response to citalopram as this antidepressant downregulates expression of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] Function: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production. Subunit: Interacts with MPDZ and INADL. May interact with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2. Subcellular Location: Cell membrane. Localizes to the post-synaptic thickening of axo-dendritic synapses. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P28223 Gene ID: 3356 Database links: Entrez Gene: 3356 Human Entrez Gene: 15558 Mouse Entrez Gene: 29595 Rat Omim: 182135 Human SwissProt: P28223 Human SwissProt: P35363 Mouse SwissProt: P14842 Rat Unigene: 654586 Human Unigene: 72630 Human Unigene: 214351 Mouse Unigene: 10294 Rat Unigene: 146756 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Small intestine (Mouse) Lysate at 40 ugPrimary: Anti-5HT2A Receptor (bs-12049R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD  Sample:Cerebellum (Mouse) Lysate at 40 ugPrimary: Anti-5HT2A Receptor (bs-12049R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD  Sample:Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-5HT2A Receptor (bs-12049R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 50 kD  Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (bs-12049R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-5HT2A Polyclonal Antibody, Unconjugated(bs-12049R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
