上海细胞库
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
| 规格 | 价格 | 库存 |
|---|---|---|
| 50ul | ￥ 980 | 200 |
| 100ul | ￥ 1680 | 200 |
| 200ul | ￥ 2480 | 200 |
| 中文名称 | 视神经视网膜相关蛋白VAX1抗体 |
| 别 名 | VAX1; VAX1_HUMAN; ventral anterior homeobox 1. |
| 研究领域 | 细胞生物 神经生物学 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=0.2μg/Test not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 35kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VAX1:133-200/334 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | The homeobox DNA-binding domain is a 60 amino acid motif that is conserved among many species and functions to bind DNA via a helix-turn-helix structure, thereby playing a role in transcriptional regulation and the control of gene expression. VAX1 (ventral anterior homeobox 1) is a 334 amino acid protein that localizes to the nucleus and contains one homeobox DNA-binding domain. Expressed as multiple alternatively spliced isoforms, VAX1 is required for major tract formation and axon guidance in the developing brain and may play a role in the differentiation of various structures, including the optic stalk, the neuroretina and the pigmented epithelium. The gene encoding VAX1 maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Function: Required for axon guidance and major tract formation in the developing forebrain. May contribute to the differentiation of the neuroretina, pigmented epithelium and optic stalk. Subcellular Location: Nucleus. DISEASE: Defects in VAX1 are the cause of microphthalmia, syndromic, type 11 (MCOPS11) [MIM:614402]. A rare clinical entity including as main characteristics microphthalmia and small optic nerves, cleft lip and palate, absence of corpus callosum, hippocampal malformations, and absence of the pineal gland. Microphthalmia is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. Similarity: Belongs to the EMX homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: Q5SQQ9 Gene ID: 11023 Database links: Entrez Gene: 11023 Human Entrez Gene: 22326 Mouse Entrez Gene: 64571 Rat Omim: 604294 Human SwissProt: Q5SQQ9 Human SwissProt: Q2NKI2 Mouse SwissProt: Q9JM00 Rat Unigene: 441536 Human Unigene: 23801 Mouse Unigene: 48764 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:MCF-7 Cell (Human) Lysate at 40 ug U937 Cell (Human) Lysate at 40 ug Primary: Anti-VAX1 (bs-11496R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 36 kD  Sample: BRL-3A Cell (Rat) Lysate at 40 ugPrimary: Anti- VAX1 (bs-11496R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 37 kD  Sample: RAW264.7 Cell (Mouse) Lysate at 40 ugPrimary: Anti- VAX1 (bs-11496R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 37 kD  Sample: NIH/3T3 Cell (Mouse) Lysate at 40 ugPrimary: Anti- VAX1 (bs-11496R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 37 kD  Blank control (blue line): Hep G2 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% methanol for 20 min at -20℃).Primary Antibody (green line): Rabbit Anti-VAX1 antibody (bs-11496R),Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test. |
