产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 蛋白偶联受体55抗体 |
| 别 名 | GPCR GPR55; G protein coupled receptor 55; G protein-coupled receptor 55; G protein-coupled receptor 55; GPCR GPR55; GPR 55; GPR55; Probable G protein coupled receptor 55; GPR55_HUMAN. |
| 研究领域 | 心血管 细胞生物 信号转导 G蛋白偶联受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Chicken, Dog, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=2ug/Test not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 37kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR55:141-240/319 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR55 is a member of this family (subfamily Orphan-A). It has been reported to be expressed in the brain, where mRNA transcripts were detected in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain. ESTs have been isolated from liver/spleen libraries. Function: May be involved in hyperalgesia associated with inflammatory and neuropathic pain. Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca(2+) release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabinoid receptor. May play a role in bone physiology by regulating osteoclast number and function. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain or in the liver. Expressed in osteoclasts and osteoblasts. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q9Y2T6 Gene ID: 9290 Database links: Entrez Gene: 9290 Human Omim: 604107 Human SwissProt: Q9Y2T6 Human Unigene: 114545 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:Lane 1: U251 (Human) Cell Lysate at 30 ug Lane 2: U2os (Human) Cell Lysate at 30 ug Primary: Anti-GPR55 (bs-7686R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD  Sample:U87MG(Human) Cell Lysate at 30 ug Primary: Anti-GPR55 (bs-7686R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD  Sample:Kidney (Mouse) Lysate at 40 ug Primary: Anti-GPR55 (bs-7686R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 37 kD  Sample: K562 Cell Lysate at 40 ugPrimary: Anti- GPR55 (bs-7686R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 35 kD  Blank control: RSC96 Cells(blue).Primary Antibody: Rabbit Anti-GPR55/AF488 Conjugated antibody (bs-7686R-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions. |
