产品分类/PRODUCT CLASSIFICATION
| 中文名称 | 蛋白磷酸酶2A抑制剂1抗体 |
| 别 名 | Acidic leucine-rich nuclear phosphoprotein 32 family member A; Acidic nuclear phosphoprotein 32 family member A; C15orf1; Acidic nuclear phosphoprotein pp32; AN32A_HUMAN; ANP32A; I1PP2A; Inhibitor 1 of protein phosphatase 2A; Lanp; Leucine Rich Acidic Nuclear Protein; Leucine-rich acidic nuclear protein; MAPM; Mapmodulin; PHAPI; Potent heat stable protein phosphatase 2A inhibitor I1PP2A; Potent heat-stable protein phosphatase 2A inhibitor I1PP2A; PP32; Putative HLA DR associated protein I; Putative HLA-DR-associated protein I; Putative Human HLA Class II Associated Protein; Putative human HLA class II associated protein I. |
| 研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 27kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PHAP1:41-140/249 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression. Subunit: Component of the SET complex, which also contains SET, APEX1, HMGB2 and NME1. Directly interacts with SET. Interacts with ATXN1/SCA1. Interacts with MAP1B (By similarity). Interacts with ELAVL1. Part of the INHAT (inhibitor of histone acetyltransferases) complex. Interacts with E4F1. Subcellular Location: Nucleus. Cytoplasm. Endoplasmic reticulum. Note=Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm. Tissue Specificity: Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala. Similarity: Belongs to the ANP32 family. Contains 4 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. SWISS: P39687 Gene ID: 8125 Database links: Entrez Gene: 8125 Human Entrez Gene: 11737 Mouse Entrez Gene: 25379 Rat Omim: 600832 Human SwissProt: P39687 Human SwissProt: O35381 Mouse SwissProt: P49911 Rat Unigene: 458747 Human Unigene: 269088 Mouse Unigene: 10123 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:293T(Human) Cell Lysate at 30 ug Primary: Anti-PHAP1 (bs-6083R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 25 kD  Sample:Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Cerebellum (Rat) Lysate at 40 ug Primary: Anti-PHAP1 (bs-6083R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (PHAP1) Polyclonal Antibody, Unconjugated (bs-6083R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (PHAP1) Polyclonal Antibody, Unconjugated (bs-6083R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (PHAP1) Polyclonal Antibody, Unconjugated (bs-6083R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. |
