产品分类/PRODUCT CLASSIFICATION
| 规格 | 价格 | 库存 |
|---|---|---|
| 100ul | ￥ 1880 | 200 |
| 200ul | ￥ 2880 | 200 |
| 中文名称 | 胰高血糖素受体抗体 |
| 别 名 | GCGR; GGR; GL R; GL-R; GLR; GLR_HUMAN; MGC138246; Glucagon receptor precursor. |
| 研究领域 | 肿瘤 细胞生物 免疫学 细胞膜受体 糖尿病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Guinea Pig, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 51kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Glucagon Receptor:11-110/477 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | The physiologic effects of glucagon are mediated through the glucagon receptor, a member of the superfamily of receptors characterized by a 7-transmembrane domain structure and by their coupling via GTP-binding proteins (G proteins) to adenyl cyclase. The glucagon receptor has been reported to be expressed in liver and adipose, but not in adrenal medulla. ESTs have been isolated from liver/spleen and kidney libraries. Function: This is a receptor for glucagon which plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in fetal tissues, including testis, adrenal gland, brain, white blood cells and brain. Similarity: Belongs to the G-protein coupled receptor 2 family. SWISS: P47871 Gene ID: 2642 Database links: Entrez Gene: 2642 Human Entrez Gene: 14527 Mouse Entrez Gene: 24953 Rat Omim: 138033 Human SwissProt: P47871 Human SwissProt: Q61606 Mouse SwissProt: P30082 Rat Unigene: 208 Human Unigene: 22329 Mouse Unigene: 11225 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Cerebellum (Mouse) Lysate at 40 ugPrimary: Anti-Glucagon Receptor (bs-3945R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD  Sample:Kidney (Mouse) Lysate at 40 ugPrimary: Anti-Glucagon Receptor(bs-3945R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51kD Observed band size: 51kD  Paraformaldehyde-fixed, paraffin embedded (human stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (bs-3945R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (bs-3945R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)).Primary Antibody: Rabbit Anti-Glucagon Receptor/AF647 Conjugated antibody (bs-3945R/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions. |
